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| Status |
Public on Apr 16, 2019 |
| Title |
Transcriptome profiling of brown adipocytes treated with BML-260, DMSO or ISO for revealing the BML-260 mechanism |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To explore BML-260 how to increase thermogenesis, we treated brown adipocytes with BML-260 and DMSO for 1 day or 3 days after seven days' differenciation. Meanwhile, we also set ISO (isoproterenol)-treated brown adipocytes as positive control. We found that BML-260 treatment led to very significant upregulation of genes involved in oxidative phosphorylation, fatty acid beta-oxidation, and mitochondrial function.When compared to cells treated with ISO, although both showed significant upregulation of UCP1, BML-260-treated cells showed a rather distinct gene profile when compared to ISO-treated cells. This work provides evidences that BML-260 can also exert a JSP-1-independent effect in activating UCP1 and thermogenesis in adipocytes, and be potentially applied to treat obesity.
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| Overall design |
Five groups of compound-treated brown adipocytes, including one-day and three-day BML-260 or DMSO treatment as well as ISO treatment. Each group has two samples.
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| Contributor(s) |
Ding Q, Feng Z, Wei Y, Zhang Y, Qiu Y, Liu X, Feng H |
| Citation(s) |
31281493 |
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| Submission date |
Apr 15, 2019 |
| Last update date |
Jul 15, 2019 |
| Contact name |
Zhuanghui Feng |
| E-mail(s) |
fengzh@sibs.ac.cn
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| Organization name |
Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences
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| Street address |
320 Yueyang Road
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| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA532837 |
| SRA |
SRP192555 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE129791_20180413_DMSO1D_BML1D_FRD0.05_1136_merge_counts.txt.gz |
53.3 Kb |
(ftp)(http) |
TXT |
| GSE129791_20180413_DMSO1D_DMSO3D_BML1D_BML3D_FRD0.05_1194_merge_counts.txt.gz |
64.5 Kb |
(ftp)(http) |
TXT |
| GSE129791_20180413_DMSO3D_BML3D_FRD0.05_3185_merge_counts.txt.gz |
146.2 Kb |
(ftp)(http) |
TXT |
| GSE129791_20180413_ISO_BML1D_BML3D_p0.05_1561_merge_counts.txt.gz |
76.6 Kb |
(ftp)(http) |
TXT |
| GSE129791_20180413_ISO_DMSO1D_DMSO3D_BML1D_BML3D_p0.05_1412_merge_counts.txt.gz |
77.1 Kb |
(ftp)(http) |
TXT |
| GSE129791_20180413_ISO_DMSO3D_BML3D_p0.05_706_merge_counts.txt.gz |
35.2 Kb |
(ftp)(http) |
TXT |
| GSE129791_BML1D_BML3D_DMSO1D_DMSO3D_ISO_all_counts.txt.gz |
389.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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