GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE129791 Query DataSets for GSE129791
Status Public on Apr 16, 2019
Title Transcriptome profiling of brown adipocytes treated with BML-260, DMSO or ISO for revealing the BML-260 mechanism
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To explore BML-260 how to increase thermogenesis, we treated brown adipocytes with BML-260 and DMSO for 1 day or 3 days after seven days' differenciation. Meanwhile, we also set ISO (isoproterenol)-treated brown adipocytes as positive control. We found that BML-260 treatment led to very significant upregulation of genes involved in oxidative phosphorylation, fatty acid beta-oxidation, and mitochondrial function.When compared to cells treated with ISO, although both showed significant upregulation of UCP1, BML-260-treated cells showed a rather distinct gene profile when compared to ISO-treated cells. This work provides evidences that BML-260 can also exert a JSP-1-independent effect in activating UCP1 and thermogenesis in adipocytes, and be potentially applied to treat obesity.
Overall design Five groups of compound-treated brown adipocytes, including one-day and three-day BML-260 or DMSO treatment as well as ISO treatment. Each group has two samples.
Contributor(s) Ding Q, Feng Z, Wei Y, Zhang Y, Qiu Y, Liu X, Feng H
Citation(s) 31281493
Submission date Apr 15, 2019
Last update date Jul 15, 2019
Contact name Zhuanghui Feng
Organization name Shanghai Institutes for Biological Sciences, University of Chinese Academy of Sciences, Chinese Academy of Sciences
Street address 320 Yueyang Road
City Shanghai
ZIP/Postal code 200031
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (10)
GSM3722140 brown adipocytes_BML1D1
GSM3722141 brown adipocytes_BML1D2
GSM3722142 brown adipocytes_DMSO1D1
BioProject PRJNA532837
SRA SRP192555

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129791_20180413_DMSO1D_BML1D_FRD0.05_1136_merge_counts.txt.gz 53.3 Kb (ftp)(http) TXT
GSE129791_20180413_DMSO1D_DMSO3D_BML1D_BML3D_FRD0.05_1194_merge_counts.txt.gz 64.5 Kb (ftp)(http) TXT
GSE129791_20180413_DMSO3D_BML3D_FRD0.05_3185_merge_counts.txt.gz 146.2 Kb (ftp)(http) TXT
GSE129791_20180413_ISO_BML1D_BML3D_p0.05_1561_merge_counts.txt.gz 76.6 Kb (ftp)(http) TXT
GSE129791_20180413_ISO_DMSO1D_DMSO3D_BML1D_BML3D_p0.05_1412_merge_counts.txt.gz 77.1 Kb (ftp)(http) TXT
GSE129791_20180413_ISO_DMSO3D_BML3D_p0.05_706_merge_counts.txt.gz 35.2 Kb (ftp)(http) TXT
GSE129791_BML1D_BML3D_DMSO1D_DMSO3D_ISO_all_counts.txt.gz 389.9 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap