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| Status |
Public on Jun 01, 2019 |
| Title |
RNA-seq assessment of quadriceps muscle and brown adipose tissue in PPARGC1A uORF-TAA mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We report a mutant mouse in which the uORF encoded by the PPARGC1A gene is disrupted via mutation of its start codon (uORF-TAA mutation). RNA-seq was used to assess differential gene expression in uORF-TAA versus wild-type mice in two metabolically important tissues that express PPARGC1A: skeletal mucle and brown adipose tissue.
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| Overall design |
mRNA was purified from quadriceps or brown adipose tissue of 10-week old male mice. Wild-type mice (n=4) and littermate uORF-TAA homozygous mice (n=4) were compared.
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| Contributor(s) |
Dumesic PA, Egan DF, Spiegelman BM |
| Citation(s) |
31105043 |
| |
| Submission date |
Apr 11, 2019 |
| Last update date |
Aug 31, 2019 |
| Contact name |
Phillip Anthony Dumesic |
| Organization name |
Dana-Farber Cancer Institute
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| Street address |
360 Longwood Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA532445 |
| SRA |
SRP192145 |
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