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| Status |
Public on Dec 10, 2019 |
| Title |
Polysome profiling RNAseq of cells transfected with an oligonucleotide targeting the ES6S region of the 40S subunit |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Polysome profiling experiments were carried out to identify the mRNAs that have altered the translation efficiency (counts in polysomal fraction/counts in monosomal fractions) in the presence of an oligonucleotide targeting a specific region of the ribosomal RNA, to study the implication of this region in the translation of specific mRNAs
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| Overall design |
Polysome profiling experiments of cuture cells transfected with the oligonucleotide of study and a control oligonucleotide with duplicates. Sequencing of the polysomal and monosomal fraction and the total RNA of each sample. There were 4 biological samples ( duplicates of cells transfected with the targeted oligonucleotide and cells tranfected with the control oligonucleotide), and 12 sequencing samples (monosomal fraction, polysomal fraction and TOTAL RNA of each biological sample)
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| Citation(s) |
31789591 |
| |
| Submission date |
Apr 11, 2019 |
| Last update date |
Dec 10, 2019 |
| Contact name |
Irene Diaz-Lopez |
| E-mail(s) |
irene.diaz@cbm.csic.es
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| Organization name |
Centro de biologia molecular Severo Ochoa
|
| Street address |
C/Nicolas Cabrera 1
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| City |
Madrid |
| ZIP/Postal code |
28049 |
| Country |
Spain |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA532392 |
| SRA |
SRP192028 |
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