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| Status |
Public on Jan 19, 2021 |
| Title |
m6A modification in effector CD4+ T cells [m6A-miCLIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Other
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| Summary |
GP61-primed effector CD4+ T cells were isolated from Ctrl or Mettl3-deficient SMARTA mice. Total RNAs were extracted with TRIzol reagent, and mRNAs were then isolated with Dynabeads® mRNA purification kit, followed by stardard m6A-miCLIP-SMARTer-seq with some modifications. Raw sequencing reads were aligned to the mouse genome (mm10) with BWA, and then m6A sites were determined.
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| Overall design |
Ctrl or Mettl3fl/flCd4-Cre SMARTA mice were primed with LCMV GP61 peptide , and 16-18 h later, CD62LloCD44hiCD4+ T cells were sorted and subjected to m6A-miCLIP-SMARTer-seq.
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| |
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| Contributor(s) |
Yao Y, Yu S |
| Citation(s) |
33637761 |
| |
| Submission date |
Apr 11, 2019 |
| Last update date |
Mar 10, 2021 |
| Contact name |
Shuyang Yu |
| E-mail(s) |
ysy@cau.edu.cn
|
| Organization name |
China Agricultural University
|
| Department |
College of Biological Sciences
|
| Street address |
2 Yuanmingyuan West Road
|
| City |
Beijing |
| ZIP/Postal code |
100193 |
| Country |
China |
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| Platforms (1) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE129650 |
m6A methyltransferase METTL3 regulates Tfh cell differentiation |
|
| Relations |
| BioProject |
PRJNA532394 |
| SRA |
SRP192027 |
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