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Series GSE129649 Query DataSets for GSE129649
Status Public on Jan 19, 2021
Title m6A modification in effector CD4+ T cells [m6A-miCLIP-seq]
Organism Mus musculus
Experiment type Other
Summary GP61-primed effector CD4+ T cells were isolated from Ctrl or Mettl3-deficient SMARTA mice. Total RNAs were extracted with TRIzol reagent, and mRNAs were then isolated with Dynabeads® mRNA purification kit, followed by stardard m6A-miCLIP-SMARTer-seq with some modifications. Raw sequencing reads were aligned to the mouse genome (mm10) with BWA, and then m6A sites were determined.
Overall design Ctrl or Mettl3fl/flCd4-Cre SMARTA mice were primed with LCMV GP61 peptide , and 16-18 h later, CD62LloCD44hiCD4+ T cells were sorted and subjected to m6A-miCLIP-SMARTer-seq.
Contributor(s) Yao Y, Yu S
Citation(s) 33637761
Submission date Apr 11, 2019
Last update date Mar 10, 2021
Contact name Shuyang Yu
Organization name China Agricultural University
Department College of Biological Sciences
Street address 2 Yuanmingyuan West Road
City Beijing
ZIP/Postal code 100193
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM4658457 Mettl3-WT_Eff-CD4-miCLIP_m6A_rep1
GSM4658458 Mettl3-WT_Eff-CD4-miCLIP_m6A_rep2
GSM4658459 Mettl3-KO_Eff-CD4-miCLIP_m6A_rep1
This SubSeries is part of SuperSeries:
GSE129650 m6A methyltransferase METTL3 regulates Tfh cell differentiation
BioProject PRJNA532394
SRA SRP192027

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Supplementary file Size Download File type/resource
GSE129649_RAW.tar 171.9 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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