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| Status |
Public on May 09, 2019 |
| Title |
Circadian gene expression in mouse gastrocnemius muscle after deletion of SIRT1 in SF1 neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Loss of synchrony between geophysical time and insulin action predisposes to metabolic diseases. Yet, the brain and peripheral pathways linking proper insulin effect to diurnal changes in light/dark and feeding/fasting inputs are poorly understood. Here, we show that insulin sensitivity of several metabolically relevant tissues fluctuates during the 24-hour period. For example, in mice insulin sensitivity of skeletal muscle, liver, and adipose tissue is lowest during the light period. Mechanistically, by performing loss- and gain-of-light-action and food-restriction experiments, we demonstrate that SIRT1 in steroidogenic factor 1 (SF1) neurons of the ventromedial hypothalamic nucleus (VMH) conveys photic inputs toentrain the biochemical and metabolic action of insulin in skeletal muscle. These findings uncover a critical light-SF1-neuron-skeletal-muscle axis that acts to finely tune diurnal changes in insulin sensitivity and reveal a light regulatory mechanism of skeletal muscle function.
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| Overall design |
Gastrocnemius muscle was dissected from 10-week old mice with deletion of SIRT1 in SF1 neurons (Sf1-Cre; Sirt1loxP/loxP) and intact controls (Sirt1loxP/loxP) at different Zeitgebers (0, 6, 12, 18).
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| Contributor(s) |
Aras E, Ramadori G, Coppari R, Corsi PS, Baldi P, Kinouchi K, Liu Y, Chen S |
| Citation missing |
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| |
| Submission date |
Apr 09, 2019 |
| Last update date |
May 09, 2019 |
| Contact name |
Siwei Chen |
| E-mail(s) |
siweic@uci.edu
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| Organization name |
University of California, Irvine
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| Department |
Computer Science
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| Lab |
Pierre Baldi
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| Street address |
66304 Verano Road South
|
| City |
irvine |
| State/province |
CA |
| ZIP/Postal code |
92617 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA531886 |
| SRA |
SRP191799 |
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