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| Status |
Public on Sep 05, 2023 |
| Title |
Transcriptional and epigenetic landscape of the mouse intestine during aging identifies key molecular drivers of aging -associated dysfunctions and diseases. [Total RNA-seq - Lgr5] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Aging is a complex multifactorial process leading to the loss of tissue/organ functionality and to an increase in disease risk. Aging-related intestinal dysfunctions include loss of barrier integrity, altered stress responses, nutrient malabsorption, and cancer formation. Many molecular mechanisms related to dysfunction and diseases are well-known (e.g. in cancer), however how aging impact on them before the occurrence of dysfunctions and diseases is poorly understood. In this study, we applied a multi-layered omics-approach to characterize the transcriptional and the epigenetic landscape of mouse intestinal epithelium during aging. We found gender and cell-type specific transcriptional and epigenetic alterations on key pathways and genes linked to intestinal dysfunctions, stem cell aging, organismal lifespan and cancer. Moreover, we identified a switch in the composition of the old intestinal stem cell subpopulations, represented by a drift towards a more secretory lineage committed (and less stem) state accompanied by functional epigenetic alterations.
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| Overall design |
Transcriptional and epigenetic landscape of the mouse intestine during aging.
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| Contributor(s) |
Krepelova A, Rasa M, Husak O, Omrani O, Sirvinskas D, Nunna S, Adam L, Käppel S, Garside G, Pourmasoumi F, Huang Y, Kries H, Rudolph KL, Neri F |
| Citation(s) |
37777550 |
| |
| Submission date |
Apr 09, 2019 |
| Last update date |
Oct 18, 2023 |
| Contact name |
Seyed Mohammd Mahdi Rasa |
| E-mail(s) |
SeyedMohammadMahdi.Rasa@uksh.de
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| Phone |
015258164616
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| Organization name |
Universitätsklinikum Schleswig-Holstein
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| Department |
Institut for Immunology
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| Lab |
Immunology (Prof. Sheffold)
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| Street address |
Michaelisstrasse 5
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| City |
Kiel |
| State/province |
Schleswig-Holstein |
| ZIP/Postal code |
24105 |
| Country |
Germany |
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| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE129713 |
Transcriptional and epigenetic landscape of the mouse intestine during aging identifies key molecular drivers of aging -associated dysfunctions and diseases |
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| Relations |
| BioProject |
PRJNA531614 |
| SRA |
SRP191511 |
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