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Series GSE129363 Query DataSets for GSE129363
Status Public on Nov 04, 2019
Title Single Cell RNASeq profiling of stromal vascular fraction from Subcutaneous and visceral adipose tissue
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The complex relationship between metabolic disease risk and body fat distribution in humans involves cellular characteristics which are specific to each body fat compartment. We applied single-cell RNA sequencing (scRNA-Seq) to identify these depot-specific differences in the stromal vascular fraction of visceral (VAT) and subcutaneous (SAT) adipose tissue of obese individuals. We characterized multiple immune cells, endothelial cells, fibroblasts, adipose and hematopoietic stem cell progenitors. Subpopulations of adipose-resident immune cells were found to be metabolically active and associated with metabolic disease status including a population of potential dysfunctional CD8+ T cells expressing metallothioneins. Adipocyte progenitors were identified at different stages of adipogenesis where a subset was pronounced in type 2 diabetics. Depot-specific analysis revealed a class of adipocyte progenitors unique to VAT possessing features similar to ‘inducible brown preadipocytes’. Our first generation human scRNA-Seq transcriptome atlas across fat depots provides a new resource to dissect functional genomics of metabolic disease.
Overall design Profiling of different cell types found in adipose derived stromal vascular fraction (SVF) of obese individuals with or without type 2 diabetes
Contributor(s) Vijay J, Grundberg E
Citation(s) 32066997
Submission date Apr 04, 2019
Last update date Feb 23, 2020
Contact name Elin Grundberg
Street address 2401 Gillham Road
City Kansas City
State/province Missouri
ZIP/Postal code 64108
Country USA
Platforms (2)
GPL20301 Illumina HiSeq 4000 (Homo sapiens)
GPL24676 Illumina NovaSeq 6000 (Homo sapiens)
Samples (32)
GSM3711757 AT-E02-SAT
GSM3711758 AT-E03-VAT
GSM3711759 AT-E07-SAT
This SubSeries is part of SuperSeries:
GSE136230 Single-cell analysis of human adipose tissue
BioProject PRJNA531020
SRA SRP190924

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE129363_Discovery_Cohort_CellAnnotation.txt.gz 136.4 Kb (ftp)(http) TXT
GSE129363_Discovery_Cohort_barcodes.tsv.gz 168.0 Kb (ftp)(http) TSV
GSE129363_Discovery_Cohort_genes.tsv.gz 258.6 Kb (ftp)(http) TSV
GSE129363_Discovery_Cohort_matrix.mtx.gz 36.8 Mb (ftp)(http) MTX
GSE129363_SVF_Normalised_Data.txt.gz 77.1 Mb (ftp)(http) TXT
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Processed data are available on Series record

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