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Series GSE129358 Query DataSets for GSE129358
Status Public on Jun 17, 2019
Title PRRX2 and HEY2 double knock-down facilitates ASCL1-induced neuron conversion in human dermal fibroblasts II
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Forced expression of ASCL1, Nurr1, Lmx1a, miRNA-124 and p53shRNA (ANLmp) in fibroblasts reprograms fibroblasts to induced dopaminergic neurons (iDA). While human lung fibroblasts can be converted rapidly and efficiently, iDA of dermal fibroblast is very unefficient and incompleted. To address this issue,  we performed time series RNAseq on both lung and dermal fibroblasts during the first several days of ANLmp induced neuron convertion. Bioinformatics analysis revealed the stable fibroblast gene regulatory network (GRN) was a potential repressive factor for iDA in human dermal fibroblasts.
Overall design Time serial comparison of transcriptomes of non-isogenic human skin fibroblasts C002 and human lung fibroblasts MRC5 during their transdifferentiation to dopaminergic neuron induced by ANLmp.
Contributor(s) Li H, Jiang H, Feng J
Citation(s) 31255287
Submission date Apr 04, 2019
Last update date Sep 16, 2019
Contact name Jian Feng
Phone 716-829-2345
Organization name State University of New York at Buffalo
Department Department of Physiology and Biophysics
Lab Feng Lab
Street address 955 Main Street, Room 3102
City Buffalo
State/province NY
ZIP/Postal code 14203
Country USA
Platforms (1)
GPL17303 Ion Torrent Proton (Homo sapiens)
Samples (8)
GSM3711717 C002_Skin Day0
GSM3711718 C002_Skin Day1
GSM3711719 C002_Skin Day3
BioProject PRJNA531004
SRA SRP190920

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Supplementary file Size Download File type/resource
GSE129358_Lung_MRC5.csv.gz 387.8 Kb (ftp)(http) CSV
GSE129358_Skin_C002.csv.gz 385.1 Kb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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