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| Status |
Public on Dec 10, 2019 |
| Title |
Rainbow trout IgM+ B cells preferentially respond to thymus-independent antigens but are activated by CD40L |
| Organism |
Oncorhynchus mykiss |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To further investigate the differential effects exerted by TD and TI-1 antigens on trout IgM+ B cells, RNA sequencing (RNA-seq) was performed on FACS isolated splenic IgM+ B cells that had been stimulated with TNP-KLH or TNP-LPS for 24 h, or on control unstimulated splenic IgM+ B cells
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| |
|
| Overall design |
9 samples; 3 biological replicates per treatment including unstimulated IgM+ B-cell as control and TNP-KLH and TNP-LPS stimulated IgM+ B-cells
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| |
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| Contributor(s) |
Granja AG, Perdiguero P, Martín-Martín A, Díaz-Rosales P, Soleto I, Tafalla C |
| Citation(s) |
31921163 |
| |
| Submission date |
Mar 31, 2019 |
| Last update date |
Jan 13, 2020 |
| Contact name |
Pedro Perdiguero |
| E-mail(s) |
pedperdi@ucm.es
|
| Phone |
916202300 2238
|
| Organization name |
CISA-INIA
|
| Department |
Animal Health Research Centre
|
| Lab |
Fish Immunology and Pathology
|
| Street address |
Ctra. de Algete a El Casar Km 8.1
|
| City |
Valdeolmos |
| State/province |
Madrid |
| ZIP/Postal code |
28130 |
| Country |
Spain |
| |
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| Platforms (1) |
| GPL19277 |
Illumina HiSeq 2000 (Oncorhynchus mykiss) |
|
| Samples (9)
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|
| Relations |
| BioProject |
PRJNA530062 |
| SRA |
SRP189955 |
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