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| Status |
Public on Mar 29, 2019 |
| Title |
Aging-exacerbated demyelinating injury is associated with microglia-derived reactive oxygen species: alleviation by indapamide |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We sequenced the transcriptome of young (6-8 week) and middle-aged (8-10 month old) C57BL/6 female mice that were naïve or had injured ventrolateral spinal cord white matter. We found several differentially expressed genes, many suggesting an altered immune response to injury with advancing age.
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| Overall design |
Naïve or injured (72 hours after the local injection of lysolecithin) ventrolateral white matter of the spinal cord was isolated using laser capture microdissection. RNA was then extracted using RNeasy MiniKit (Qiagen) as per manufacturer’s instruction and sequenced using the Illumina NextSeq500. Tissue was pooled from 5 animals per condition and 3 replicates were sequenced for the analysis.
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| Contributor(s) |
Michaels NJ, Lemmon K, Plemel JR, Jensen SK, Brown D, Mishra MK, Rawji KS, Koch M, Yong VW |
| Citation(s) |
32030468 |
| |
| Submission date |
Mar 28, 2019 |
| Last update date |
Feb 14, 2020 |
| Contact name |
V. Wee Yong |
| E-mail(s) |
vyong@ucalgary.ca
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| Organization name |
University of Calgary
|
| Street address |
3330 Hospital Dr. NW
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| City |
Calgary |
| State/province |
AB |
| ZIP/Postal code |
T2N 4N1 |
| Country |
Canada |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA529670 |
| SRA |
SRP189771 |
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