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| Status |
Public on Oct 29, 2019 |
| Title |
Genetically diverse uropathogenic Escherichia coli adopt a common transcriptional program in patients with urinary tract infections |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Uropathogenic Escherichia coli (UPEC) is the major causative agent of uncomplicated urinary tract infections (UTIs). A common virulence genotype of UPEC strains responsible for UTIs is yet to be defined, due to the large variation of virulence factors observed in UPEC strains. We hypothesized that studying UPEC functional responses in patients might reveal universal UPEC features that enable pathogenesis. Here we identify a transcriptional program shared by genetically diverse UPEC strains isolated from 14 patients during uncomplicated UTIs. Strikingly, this in vivo gene expression program is marked by upregulation of translational machinery, providing a mechanism for the rapid growth within the host. Our analysis indicates that switching to a more specialized catabolism and scavenging lifestyle in the host allows for the increased translational output. Our study identifies a common transcriptional program underlying UTIs and illuminates the molecular underpinnings that likely facilitate the fast growth rate of UPEC in infected patients.
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| Overall design |
14 UPEC mRNA profiles isolated directly from patient urine (collected into RNAprotect), and 14 UPEC mRNA profiles from in vitro cultures in filter-sterilized urine (mid-log phase)
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| Contributor(s) |
Sintsova A, Frick-Cheng A, Smith S, Pirani A, Snitkin E, Mobley H |
| Citation(s) |
31633483 |
| |
| Submission date |
Mar 28, 2019 |
| Last update date |
Oct 29, 2019 |
| Contact name |
Harry Mobley |
| E-mail(s) |
hmobley@med.umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Microbiology and Immunology
|
| Street address |
1150 W. Medical Center Drive
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18133 |
Illumina HiSeq 2500 (Escherichia coli) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA529666 |
| SRA |
SRP189765 |
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