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| Status |
Public on Jul 31, 2020 |
| Title |
T cell regeneration from pluripotent stem cells by defined transcription factors Runx1 and Hoxa9 [single hematopoietic cells (iR9-iHEC&iR9-iHPC) II] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We identified that the coordinated expression of exogenous Runx1 and Hoxa9 during the haematopoietic induction process from mouse PSC resulted in a type of induced hemogenic endothelial cells (iHEC). The single iHEC can be robustly educated into induced haematopoietic progenitor cells (iHPC), which gave rise to induced T cells (iT cells) with abundant TCRαβ repertoire in vivo. We sorted the single iHEC and iHPC from day-14, day-17, day-21 cell products derived from 241 iR9-ES and performed single-cell RNA-Seq.
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| Overall design |
mRNA profiles of single hematopoietic endothelia cell (iHEC) or hematopoietic progenitor cell. single cells were sorted from PSC-derived cells for RNAseq analysis according to kit instruction of Discover-sc WTA Kit V2(Vazyme) and TruePrepTM DNA Library Prep Kit V2 (Vazyme), using illumina HiSeq X Ten.
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| Contributor(s) |
Weng Q, Wang J |
| Citation(s) |
31729468 |
| |
| Submission date |
Mar 22, 2019 |
| Last update date |
Nov 07, 2020 |
| Contact name |
Jinyong Wang |
| E-mail(s) |
wang_jinyong@gibh.ac.cn
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| Organization name |
Guangzhou Institutes of Biomedicine and Health (GIBH)
|
| Street address |
190 Kai Yuan Avenue, Science Park, Guangzhou, China
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| City |
Guangzhou |
| State/province |
GuangDong |
| ZIP/Postal code |
510530 |
| Country |
China |
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| Platforms (1) |
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| Samples (160)
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| This SubSeries is part of SuperSeries: |
| GSE121375 |
T cell regeneration from pluripotent stem cells by defined transcription factors |
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| Relations |
| BioProject |
PRJNA528652 |
| SRA |
SRP189197 |
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