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Series GSE128730 Query DataSets for GSE128730
Status Public on Jan 07, 2020
Title RNAseq of parental and neratinib resistant 5637 and OVCAR8 cells with or withour neratinib treatment
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Pan-HER TKI neratinib has demonstrated clinical activity in patients with HER2-mutant cancers. However responses are heterogenoeus and not generally prolonged, suggesting de-novo and acquired resistance to neratinib. To study mechanisms of resistance to neratinib we generated neratinib resistant cells by gradual dose escalation until resistance was achieved and performed various analyses including RNAseq.
Overall design 5637NR and OVCAR8NR cells were maintained under drug-free conditions for 1 week prior to seeding. Parental and neratinib-resistant cells were seeded in triplicate in 10-cm dishes and then treated with or without neratinib (5637 - 600 nM; OVCAR8 - 1 μM); 24h later, cells were harvested and RNA was purified using ReliaPrep RNA Cell Miniprep system (Promega).
Contributor(s) Arteaga CL
Citation(s) 31978326
Submission date Mar 22, 2019
Last update date Apr 07, 2020
Contact name Dhivya Sudhan
Organization name Vanderbilt University
Department Hematology/Oncology Division
Street address 777 Preston Research Building
City Nashville
State/province TN
ZIP/Postal code 37232-6307
Country USA
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (24)
GSM3683927 5637 parental control-1 [272 DS-1]
GSM3683928 5637 parental control-2 [272 DS-2]
GSM3683929 5637 parental control-3 [272 DS-3]
BioProject PRJNA528648
SRA SRP189178

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128730_RNAseq.count.txt.gz 1.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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