|
| Status |
Public on Jul 23, 2019 |
| Title |
EGFR and hair shaft differentiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
EGFR signaling controls skin development and homeostasis in mice and humans and its deficiency causes severe skin inflammation, which might affect epidermal stem cell behaviour. Here, we describe the inflammation-independent effects of EGFR-deficiency during skin morphogenesis and in adult HFSCs. Expression and alternative splicing analysis of RNAseq data from interfollicular epidermis and outer root sheath indicate that EGFR controls genes involved in epidermal differentiation, but also in centrosome function, DNA damage, cell cycle and apoptosis. Genetic experiments employing p53-deletion in EGFR–deficient epidermis reveal that EGFR signalling exhibits p53-dependent functions in proliferative epidermal compartments, as well as p53–independent functions in differentiated hair shaft keratinocytes. Loss of EGFR leads to absence of Lef1 protein specifically in the innermost epithelial hair layers, resulting in disorganization of medulla cells. Thus, our results uncover important spatial and temporal features of cell-autonomous EGFR functions in the epidermis.
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| |
|
| Overall design |
150bp PE RNA-seq, EGFR knock out vs controls
|
| |
|
| Contributor(s) |
Amberg N |
| Citation(s) |
31082735 |
| |
| Submission date |
Mar 18, 2019 |
| Last update date |
Sep 25, 2023 |
| Contact name |
Gerwin Heller |
| E-mail(s) |
gerwin.heller@meduniwien.ac.at
|
| Organization name |
Medical University of Vienna
|
| Department |
Medicine I
|
| Street address |
Währinger Gürtel 18-20
|
| City |
Wien |
| ZIP/Postal code |
1090 |
| Country |
Austria |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA527731 |
| SRA |
SRP188688 |
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