NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE128436 Query DataSets for GSE128436
Status Public on Jul 23, 2019
Title EGFR and hair shaft differentiation
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary EGFR signaling controls skin development and homeostasis in mice and humans and its deficiency causes severe skin inflammation, which might affect epidermal stem cell behaviour. Here, we describe the inflammation-independent effects of EGFR-deficiency during skin morphogenesis and in adult HFSCs. Expression and alternative splicing analysis of RNAseq data from interfollicular epidermis and outer root sheath indicate that EGFR controls genes involved in epidermal differentiation, but also in centrosome function, DNA damage, cell cycle and apoptosis. Genetic experiments employing p53-deletion in EGFR–deficient epidermis reveal that EGFR signalling exhibits p53-dependent functions in proliferative epidermal compartments, as well as p53–independent functions in differentiated hair shaft keratinocytes. Loss of EGFR leads to absence of Lef1 protein specifically in the innermost epithelial hair layers, resulting in disorganization of medulla cells. Thus, our results uncover important spatial and temporal features of cell-autonomous EGFR functions in the epidermis.
 
Overall design 150bp PE RNA-seq, EGFR knock out vs controls
 
Contributor(s) Amberg N
Citation(s) 31082735
Submission date Mar 18, 2019
Last update date Sep 25, 2023
Contact name Gerwin Heller
E-mail(s) gerwin.heller@meduniwien.ac.at
Organization name Medical University of Vienna
Department Medicine I
Street address Währinger Gürtel 18-20
City Wien
ZIP/Postal code 1090
Country Austria
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM3674810 E341_FC7
GSM3674811 E341_FC8
GSM3674812 E344
Relations
BioProject PRJNA527731
SRA SRP188688

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE128436_RAW.tar 5.0 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap