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| Status |
Public on Oct 12, 2019 |
| Title |
The piRNA Response to Retroviral Invasion of the Koala Genome |
| Organisms |
Mus musculus; Phascolarctos cinereus |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Other
|
| Summary |
Antisense piRNAs guide silencing of established transposons during germline development, and sense piRNAs drive ping-pong amplification of the antisense pool, but how the germline responds to genome invasion is not understood. The KoRV-A gammaretrovirus infects the soma and germline and is sweeping through wild koalas by a combination of horizontal and vertical transfer, allowing direct analysis of retroviral invasion of the germline genome. Gammaretroviruses produce spliced Env mRNAs and unspliced transcripts encoding Gag, Pol and the viral genome, but KoRV-A piRNAs are almost exclusively derived from unspliced genomic transcripts and are strongly sense strand biased. Significantly, selective piRNA processing of unspliced proviral transcripts is conserved from insects to placental mammals. We speculate that bypassed splicing generates a conserved molecular pattern that directs proviral genomic transcripts to the piRNA biogenesis machinery, and that this “innate” piRNA response suppresses transposition until antisense piRNAs are produced, establishing sequence-specific adaptive immunity.
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| Overall design |
DNAseq, RNAseq and small RNAseq data for koala testes, livers and brains; RNAseq and small RNAseq data for different strains of mouse.
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| Contributor(s) |
Yu T, Koppetsch B, Weng Z, Theurkauf WE, Keith C, Jeremy L |
| Citation(s) |
31607510 |
| |
| Submission date |
Mar 11, 2019 |
| Last update date |
May 31, 2020 |
| Contact name |
Tianxiong Yu |
| Organization name |
Umass Med
|
| Street address |
364 Plantation St
|
| City |
Worcester |
| State/province |
MA |
| ZIP/Postal code |
01605 |
| Country |
USA |
| |
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| Platforms (4)
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| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24665 |
Illumina NextSeq 500 (Phascolarctos cinereus) |
| GPL26281 |
Illumina NovaSeq 6000 (Phascolarctos cinereus) |
| GPL26282 |
Illumina HiSeq 4000 (Phascolarctos cinereus) |
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| Samples (31)
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| Relations |
| BioProject |
PRJNA526502 |
| SRA |
SRP188109 |
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