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| Status |
Public on Aug 24, 2021 |
| Title |
Expression data from mouse heart post transverse aortic constriction (TAC) -surgery 2 weeks |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
A number of mediators that have been found to be involved in the pathogenesis of cardiac hypertrophy affecting gene transcription, protein synthesis, metabolism,autophagy, oxidative stress and inflammation. These mediators including nuclear transcription factor, microRNAs and long noncoding RNAs. However, the gene expression profiles signaling pathways in the pressure overload-induced cardiac hypertrophy remain unknown.
All mice were randomly undertaken sham surgery or TAC for 2 weeks. Hearts were harvested 2 weeks following TAC and performed to further analysis.
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| Overall design |
For microarray profiling, the mice heart total RNA was harvested using TRIzol reagent (Invitrogen, Carlsbad, Canada) and purified using RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s protocol. The amount and quality of RNA were determined using UV-Vis Spectrophotometer (Thermo, NanoDrop 2000, USA) at the absorbance of 260 nm. The mRNA expression profiling was measured using Affymetrix ClariomTM D mouse Array (Affymetrix GeneChip, Santa Clara CA, USA), which contains 65957 gene-level probe sets.
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| Contributor(s) |
Qu A, Wang X |
| Citation(s) |
34376812 |
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| Submission date |
Mar 06, 2019 |
| Last update date |
Aug 25, 2021 |
| Contact name |
Xia Wang |
| E-mail(s) |
wangxia@ccmu.edu.cn
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| Organization name |
capital medical university
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| Department |
Department of Physiology and Pathophysiology
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| Street address |
No.10 Xitoutiao, You An Men
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| City |
Beijing |
| ZIP/Postal code |
100069 |
| Country |
China |
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| Platforms (1) |
| GPL20775 |
[MTA-1_0] Affymetrix Mouse Transcriptome Array 1.0 [transcript (gene) CSV version] |
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| Samples (16)
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| GSM3657212 |
N_S2W, biological rep4 |
| GSM3657213 |
N_T2W, biological rep1 |
| GSM3657214 |
N_T2W, biological rep2 |
| GSM3657215 |
N_T2W, biological rep3 |
| GSM3657216 |
N_T2W, biological rep4 |
| GSM3657217 |
P_S2W, biological rep1 |
| GSM3657218 |
P_S2W, biological rep2 |
| GSM3657219 |
P_S2W, biological rep3 |
| GSM3657220 |
P_S2W, biological rep4 |
| GSM3657221 |
P_T2W, biological rep1 |
| GSM3657222 |
P_T2W, biological rep2 |
| GSM3657223 |
P_T2W, biological rep3 |
| GSM3657224 |
P_T2W, biological rep4 |
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| Relations |
| BioProject |
PRJNA525864 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE127954_RAW.tar |
330.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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