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| Status |
Public on Jun 18, 2019 |
| Title |
Induction of Pluripotency by Alternative Factors (RNA-Seq) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Reprogramming somatic cells to pluripotency represents a paradigm for cell fate determination. A binary logic of closing and opening chromatin provides a simple way to understand iPSC reprogramming driven by both Yamanaka factors or chemicals. Here we apply this logic to the design a seven factor combination, Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Essrb and Sall4 (7F), that reprogram MEFs to chimera competent iPSCs efficiently. RNA- and ATAC-seq reveal differences between 7F and Yamanaka induced pluripotency, 7IP and YIP, in transcriptomic and chromatin accessibility dynamics(CAD). Sall4 emerges as a dominant force that can close and open chromatin with the help of Jdp2 and Glis1 in resetting somatic chromatin to a pluripotent state. These results reveal a previously unknown path between somatic and pluripotent states, open a door for cell fate control.
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| Overall design |
Seven factor combination, Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Essrb and Sall4 (7F)
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| Contributor(s) |
Wang B |
| Citation(s) |
31216469 |
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| Submission date |
Mar 06, 2019 |
| Last update date |
Jun 20, 2022 |
| Contact name |
dongwei Li |
| E-mail(s) |
li_dongwei@gibh.ac.cn
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| Organization name |
Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
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| Department |
South China Institute for Stem Cell Biology and Regenerative Medicine
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| Lab |
Duanqing Pei lab
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| Street address |
190 Kai Yuan Avenue, Science Park
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| City |
Guangzhou |
| State/province |
Guangdong province |
| ZIP/Postal code |
510530 |
| Country |
China |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (68)
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| Relations |
| BioProject |
PRJNA525825 |
| SRA |
SRP187722 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE127927_RAW.tar |
75.4 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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