Expression profiling by high throughput sequencing
Summary
The hypothesis is that HIV affects TRM, by decreasing their numbers and possibly affecting their function. In order to be able to analyse several HIV+ and HIV- samples, we would need to identify some markers for TRM that can be used for flow cytometry-based detection of TRM.
Overall design
To characterize TRM, we will use a RNA seq approach, in order to identify new markers for CD8+ TRM in human tissues. We will compare CD69+, CD69+ CD103+ and CD69- CD103- populations in the tissue and effector memory T cells, as well as CD69+ T cells in the blood.
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