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Series GSE127662 Query DataSets for GSE127662
Status Public on Mar 01, 2022
Title lncRNA expression profiles in spermatocyte cell line (GC2) after exposure to triptolide and triptonide
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Triptolide or triptonide, major bioactive components isolated from the traditional Chinese herb Tripterygium wilfordii Hook f. (TWHF), have been shown to exert various pharmacological effects on systemic autoimmune disease. However, the severe toxicity, especially reproduction toxicity, of triptolide or triptonide prevents wide clinical use for those people with fertility needs. Long noncoding (lnc) RNAs are critical for spermatogenesis, and their dysregulation might lead to male infertility. However, whether they are involved in triptolide or triptonide -induced reproduction toxicity is unknown. Here we found that exposure of spermatocyte cell line (GC2) to triptolide or triptonide led to reduction in cell viability and induced cell apoptosis and autophagy. Then the total RNAs of triptolide or triptonide -treated GC2 cells were used to investigate lncRNA expression profiles by strand-specific RNA sequencing at the transcriptome level to help determine any RNA-related mechanisms in triptolide or triptonide-induced toxicity. Gene ontology and pathway analyses showed that the functions of differentially expressed lncRNA targets and mRNAs were closely linked with many processes involved in spermatogenesis.
Overall design LncRNA profiles of testis were generated by stranded-specific RNA-seq, using Illumina HiSeqâ„¢ X Ten platform with PE150.
Contributor(s) Zhang X, Xiong S
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Submission date Mar 01, 2019
Last update date Mar 01, 2022
Contact name Xiaoning Zhang
Organization name Nanchang University
Street address 999 Xuefu R.D., Nanchang
City Nanchang
ZIP/Postal code 330031
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (6)
GSM3636320 Con_1
GSM3636321 Con_2
GSM3636322 Tiptolide_1
BioProject PRJNA525002
SRA SRP187178

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Supplementary file Size Download File type/resource
GSE127662_lncRNA.fa.gz 173.0 Kb (ftp)(http) FA
GSE127662_transcript_FPKM.xls.gz 3.4 Mb (ftp)(http) XLS
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Raw data are available in SRA
Processed data are available on Series record

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