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| Status |
Public on Feb 28, 2019 |
| Title |
Mating enhances expression of hormonal and trophic factors in the midbrain of female rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Among female Long-Evans rats, mating enhances neurosteroid formation in the midbrain ventral tegmental area (independent of peripheral steroid-secreting glands, the ovaries and adrenals). The sources/targets for these actions are not well-understood. In Experiment 1, proestrous rats engaged in a mating paradigm, or did not, and had midbrains assessed via Affymetrix rat genome microarrays. In Experiment 2, the influence of gonadal and adrenal glands on the expression of these genes was assessed in rats that were proestrous, ovariectomized (OVX), or OVX and adrenalectomized (ADX).
Microarrays revealed 53 target genes that were significantly up-regulated (> 2.0-fold change) in response to mating. Mating significantly enhanced midbrain mRNA expression of genes involved in hormonal and trophic actions: Gh1, S100g, and Klk1b3 in proestrous, but not OVX and/or ADX, rats; Fshb in all but OVX/ADX rats; and Lhb and Tshb in all rats. Thus, mating enhances midbrain gene expression, independent and dependent of, peripheral glands.
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| Overall design |
Experiment 1: proestrous rats engaged in a mating paradigm, or did not, and had midbrains assessed via Affymetrix rat genome 2.0 microarrays. In Experiment 2, the influence of gonadal and adrenal glands on the expression of these genes was assessed in rats that were proestrous, ovariectomized (OVX), or OVX and adrenalectomized (ADX).
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| Contributor(s) |
Frye CA, Tine JA, Chittur SV |
| Citation(s) |
32351369 |
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| Submission date |
Feb 27, 2019 |
| Last update date |
May 07, 2020 |
| Contact name |
Sridar V Chittur |
| E-mail(s) |
schittur@albany.edu
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| Phone |
518-591-7215
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| Organization name |
SUNY-University at Albany
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| Department |
Center for Functional Genomics
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| Lab |
Microarray Core
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| Street address |
One Discovery Drive, CRC 342G
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| City |
Rensselaer |
| State/province |
NY |
| ZIP/Postal code |
12144 |
| Country |
USA |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA524646 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE127272_RAW.tar |
68.9 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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