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| Status |
Public on Feb 28, 2019 |
| Title |
Effect of sulforaphane and 5-aza-2'-deoxycytidine on melanoma cell growth |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: UV exposure-induced oxidative stress is implicated as the driving mechanism for melanoma. Increased oxidative stress results in DNA damage and epigenetic modifications. We wondered whether a low dose of antioxidant could attenuate the oxidative stress of and help cells respond to epigenetic modification treatment at a lower dose. Sulforaphane (SFN) is a natural bioactivated product of the cruciferous family and is known as an antioxidant. We investigated the combinational effect of SFN and epigenetic modification drug, 5-aza-2'-deoxycytidine (DAC), on metastatic melanoma growth.
Methods: Cell growth characteristics, RNA-seq and histone post-translational modification markers (PTMs) were compared in single and combination treatments.
Results: We found increased cell growth inhibition and changes in gene expression profiles which includes a key immuno-regulator cytokine, C-C motif ligand 5 (CCL-5) gene expression upon combinational treatments. There was no significant difference in detectable histone PTMs between treatments.
Conclusions: These results indicate a potential combinational effect of a dietary antioxidant and an FDA-approved epigenetic drug in controlling melanoma growth. The long-term goal of the current study is to find the therapeutic role of the dietary antioxidant SFN as a supporting drug for targeted epigenetic treatment with DAC in controlling melanoma growth.
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| Overall design |
mRNA profiles of mouse B16F10 melanoma cells treated with DMSO (control) , DAC, SFN, or combination treatment (DAC + SFN) were generated by deep sequencing using illumina's NextSeq 500 instrument (75bp SE reads). Three independent biological repeats were performed for all sample groups.
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| Contributor(s) |
Chiang T, Koss BS, Su LJ, Washam CL, Byrum SD, Storey A, Tackett AJ |
| Citation(s) |
31252639 |
| |
| Submission date |
Feb 27, 2019 |
| Last update date |
Oct 21, 2019 |
| Contact name |
Ravi W. Sun |
| E-mail(s) |
RSun@uams.edu
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| Organization name |
University of Arkansas for Medical Sciences
|
| Department |
Department of Otolaryngology - Head and Neck Surgery
|
| Street address |
4301 W Markham St
|
| City |
Little Rock |
| State/province |
Arkansas |
| ZIP/Postal code |
72205 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA524486 |
| SRA |
SRP187004 |
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