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Series GSE127119 Query DataSets for GSE127119
Status Public on Sep 11, 2019
Title Variant PRC2.1 and PRC2.2 co-operate to direct H3K27 methylations in ESCs [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Polycomb repressive complex 2 (PRC2) is composed of EED, SUZ12, and EZH1/2, and mediates mono-, di- and tri-methylation of Histone H3 at Lysine 27. While, two subcomplexes defined by their specific accessory proteins, termed PRC2.1 (Polycomb-like proteins 1-3, EPOP and PALI1/2) and PRC2.2 (AEBP2 and JARID2) exist, little is known about their differential functions. Here, we show that PRC2.1 and PRC2.2 share the majority of their target genes in mouse embryonic stem cells (ESCs). While loss of all three Polycomb-like proteins (PHF1, MTF2, PHF19) leads to eviction of EPOP and PALI1 from chromatin, PRC2.2 specific components are maintained together with SUZ12 and H3K27me3 at broad Polycomb domains. We show that PRC2.2 localises to PRC1 mediated H2AK119ub1 at these broad Polycomb domains to maintain H3K27me3, even in the absence of PRC2.1. Taken together, these data establish that PRC2.1 and PRC2.2 co-operate to direct H3K27me3, but have independent recruitment mechanisms.
Overall design RNA seq analysis of Pcl1-3 WT and Pcl1-3 TKO mouse embryonic stem cells and at three time points (Day 2, Day 4 and Day 8) during embryoid body differentiation (EB). 20 samples are included. Biological duplicates are present.
Contributor(s) Healy E, Mucha M, Fitzpatrick DJ, Conway E, Van Mierlo G, Baltissen MP, Vermeulen M, Koseki Y, Koseki H, Bracken AP
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Submission date Feb 25, 2019
Last update date Sep 13, 2019
Contact name Darren John Fitzpatrick
Organization name Trinity College Dublin
Department Smurfit Institute of Genetics
Street address College Green
City Dublin 2
State/province Ireland
ZIP/Postal code Eire
Country Ireland
Platforms (1)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (20)
GSM3625712 WT_ESC_Ser_LIF_rep1
GSM3625713 KO_ESC_Ser_LIF_rep1
GSM3625714 WT_ESC_Ser_LIF_rep2
This SubSeries is part of SuperSeries:
GSE127121 Variant PRC2.1 and PRC2.2 co-operate to direct H3K27 methylations in ESCs
BioProject PRJNA524182
SRA SRP186798

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE127119_RAW.tar 1.5 Gb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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