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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Sep 11, 2019 |
| Title |
Variant PRC2.1 and PRC2.2 co-operate to direct H3K27 methylations in ESCs [RNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Polycomb repressive complex 2 (PRC2) is composed of EED, SUZ12, and EZH1/2, and mediates mono-, di- and tri-methylation of Histone H3 at Lysine 27. While, two subcomplexes defined by their specific accessory proteins, termed PRC2.1 (Polycomb-like proteins 1-3, EPOP and PALI1/2) and PRC2.2 (AEBP2 and JARID2) exist, little is known about their differential functions. Here, we show that PRC2.1 and PRC2.2 share the majority of their target genes in mouse embryonic stem cells (ESCs). While loss of all three Polycomb-like proteins (PHF1, MTF2, PHF19) leads to eviction of EPOP and PALI1 from chromatin, PRC2.2 specific components are maintained together with SUZ12 and H3K27me3 at broad Polycomb domains. We show that PRC2.2 localises to PRC1 mediated H2AK119ub1 at these broad Polycomb domains to maintain H3K27me3, even in the absence of PRC2.1. Taken together, these data establish that PRC2.1 and PRC2.2 co-operate to direct H3K27me3, but have independent recruitment mechanisms.
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| Overall design |
RNA seq analysis of Pcl1-3 WT and Pcl1-3 TKO mouse embryonic stem cells and at three time points (Day 2, Day 4 and Day 8) during embryoid body differentiation (EB). 20 samples are included. Biological duplicates are present.
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| Contributor(s) |
Healy E, Mucha M, Fitzpatrick DJ, Conway E, Van Mierlo G, Baltissen MP, Vermeulen M, Koseki Y, Koseki H, Bracken AP |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Feb 25, 2019 |
| Last update date |
Sep 13, 2019 |
| Contact name |
Darren John Fitzpatrick |
| E-mail(s) |
fitzpadj@tcd.ie
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| Organization name |
Trinity College Dublin
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| Department |
Smurfit Institute of Genetics
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| Street address |
College Green
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| City |
Dublin 2 |
| State/province |
Ireland |
| ZIP/Postal code |
Eire |
| Country |
Ireland |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (20)
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| This SubSeries is part of SuperSeries: |
| GSE127121 |
Variant PRC2.1 and PRC2.2 co-operate to direct H3K27 methylations in ESCs |
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| Relations |
| BioProject |
PRJNA524182 |
| SRA |
SRP186798 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE127119_RAW.tar |
1.5 Gb |
(http)(custom) |
TAR (of BW) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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