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| Status |
Public on Feb 28, 2019 |
| Title |
Mice VSMCs: Control vs. TNF-α cultured |
| Organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Transcriptional profiling of mouse VSMCs comparing control with VSMCs cultured with TNF-α
Apoptosis of vascular smooth muscle cells (VSMCs) is a process that regulates vessel remodeling in various cardiovascular diseases. The specific mechanisms that control VSMC apoptosis remain unclear. The present study aimed to investigate whether microRNA-494 (miR-494) is involved in regulating VSMC apoptosis and its underlying mechanisms. Cell death ELISA and terminal deoxynucleotidyl-transferase-mediated dUTP nick end labeling assays were used to detect apoptosis of murine VSMCs following stimulation with tumor necrosis factor(TNF-α). The results indicated that TNF-α-upregulated VSMC apoptosis in a dose-dependent manner. Microarray analysis was used to evaluate the expression profile of microRNAs following TNF-α-stimulation in murine VSMCs. The expression of miR-494 was downregulated, whereas B-cell lymphoma 2-like 11 (BCL2L11) protein expression levels were upregulated in VSMCs following treatment with TNF- . Luciferase reporter assays confirmed that BCL2L11 was a direct target of miR-494. Transfection with miR-494 mimics decreased VSMC apoptosis and downregulated BCL2L11 protein levels. Conversely, transfection with miR-494 inhibitors increased cell apoptosis and upregulated BCL2L11 protein levels, suggesting that miR-494 may function as an essential regulator of BCL2L11. The increase in apoptosis caused by miR-494 inhibitors was abolished in cells co-transfected with BCL2L11-targeting small interfering RNA. The findings of the present study revealed that miR-494 inhibited TNF-α-induced VSMC apoptosis by downregulating the expression of BCL2L11.
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| Overall design |
Two-condition experiment, Control vs.TNF-α cultured VSMCs . Biological replicates: 3 control, 3 TNF-α cultured VSMCs, independently grown and harvested.
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| Contributor(s) |
Cui R, Yi L |
| Citation missing |
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| |
| Submission date |
Feb 25, 2019 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Rongrong Cui |
| E-mail(s) |
rongrongcui@csu.edu.cn
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| Organization name |
The second Xiangya hospital of central south university
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| Street address |
139，renming road
|
| City |
hunan |
| ZIP/Postal code |
410000 |
| Country |
China |
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| Platforms (1) |
| GPL26224 |
LC_MRA-2001_Mouse miRNA Array [miRMouse_17_SXH_110728] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA524301 |
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