|
| Status |
Public on Apr 21, 2020 |
| Title |
Genome-wide methyl-cytosine competition by DNMT and TET |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
We define distinct methylated elements that recruit both DNMT3A/B and TETs. We also show the genome-wide activity of DNMTs and TETs.
|
| |
|
| Overall design |
We performed WGBS for a series of HUES64 and HUES8 human embryonic stem cells: wildtype (WT), DNMT3A and DNMT3B double knockout (DKO), TET triple knockout (TKO), TKO plus DNMT3B knockout (QKO) and QKO plus DNMT3A knockout (PKO). We also performed bulk RNA sequencing.
|
| |
|
| Contributor(s) |
Charlton J, Meissner A |
| Citation(s) |
32514123 |
| |
| Submission date |
Feb 22, 2019 |
| Last update date |
Jul 21, 2020 |
| Contact name |
Jocelyn Charlton |
| E-mail(s) |
jocelyn_charlton@harvard.edu
|
| Organization name |
Harvard University
|
| Department |
Stem Cell and Regenerative Biology
|
| Lab |
Meissner Lab
|
| Street address |
7 Divinity Avenue
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platforms (4)
|
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (42)
|
|
| Relations |
| BioProject |
PRJNA523851 |
| SRA |
SRP186642 |
Less...
More...