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| Status |
Public on Jan 30, 2020 |
| Title |
RNA-seq analysis of high fat diet-induced changes in macrophage transcriptome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Systemic low-grade inflammation associated with obesity and the metabolic syndrome is also a strong risk factor for the development of diabetes. This inflammatory state is primarily caused by the release of proinflammatory cytokines by macrophages. However, the effect of obesity on the macrophage transcriptome, including long non-coding RNAs, is not known. Here, we analyzed global changes in gene expression of elicited peritoneal macrophages from mice fed a high-fat diet (HFD) or control diet for 12 weeks. The HFD-fed mice has significantly increased body weight and impaired glucose tolerance compared to controls. Transcriptome analysis showed that HFD incuced the expression of genes associated with inflammatory response.
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| Overall design |
C57BL/6J mice were fed with standard chow or high-fat diet (HFD, 60%kcal, Research Diets Inc., D12492i) for 12 weeks. Mice were administered with 3% thioglycollate medium via intraperitoneal injection, and four days later, peritoneal macrophages were collected.
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| Contributor(s) |
Stapleton K, Natarajan R |
| Citation(s) |
32078363 |
| |
| Submission date |
Feb 21, 2019 |
| Last update date |
Apr 13, 2020 |
| Contact name |
Kenneth Edmund Stapleton |
| E-mail(s) |
kstapleton@coh.org
|
| Organization name |
City of Hope
|
| Department |
Diabetes Complications and Metabolism
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| Lab |
Natarajan Lab
|
| Street address |
1500 Duarte Rd
|
| City |
Duarte |
| State/province |
CA |
| ZIP/Postal code |
91010 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA523618 |
| SRA |
SRP186510 |
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