 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Mar 01, 2020 |
| Title |
Human ESC-embryoid body sorted populations: TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Signal differences between TMRM-hi and ALCAM-low vs. TMRM-hi and ALCAM-hi in representative cardiac and hepatic gene expression in mRNA microarray analysis.
INTRODUCTION: Non-genetic purification methods for pluripotent stem cell-derived hepatocyte-like cells are useful for liver regenerative therapy and pharmaceutical applications. METHODS: Fluorescent activated cell sorting (FACS) was used to separate cells by combining two parameters: cellular mitochondrial content evaluated by the mitochondrial membrane potential-dependent fluorescent probe (TMRM) and immunocytochemical detection of activated leukocyte cell adhesion molecule (ALCAM). This method was applied to murine fetal, human embryonic stem cell (ESC)-derived, and human induced pluripotent stem cell (iPSC)-derived cell-mixtures. Separately sorted cell fractions were evaluated by quantitative PCR, immunohistochemistry, and cytochemistry for HNF4a, AFP, and albumin mRNA and/or protein expression. RESULTS: Hepatocyte-like cells were highly segregated into the high TMRM signal and ALCAM-positive population. The purity of hepatocyte-like cells derived from human iPSCs was 97 ± 0.38% (n = 5). CONCLUSIONS: This hepatocyte-like cell purification method may be applicable to quality control of cells for liver regenerative cell therapy and pharmaceutical development.
|
| |
|
| Overall design |
comparison of each one sample
|
| |
|
| Contributor(s) |
Yamashita H, Hattori F |
| Citation(s) |
33615028 |
| |
| Submission date |
Feb 20, 2019 |
| Last update date |
Apr 07, 2021 |
| Contact name |
Satoshi Kondo |
| Organization name |
Toray Industries,Inc.
|
| Department |
New Projects Development Division
|
| Street address |
Tebiro 6-10-1
|
| City |
Kamakura |
| State/province |
Kanagawa |
| ZIP/Postal code |
248-8555 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL13915 |
3D-Gene Human Oligo chip 25k V2.1 |
|
| Samples (1) |
|
| Relations |
| BioProject |
PRJNA523383 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE126812_RAW.tar |
820.0 Kb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
|
|
|
|
 |
