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| Status |
Public on Nov 14, 2022 |
| Title |
IL-1 receptor antagonist prevents bias to myelopoiesis in hematopoietic stem cells and protects from neoplasia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Interleukin-1β (IL-1β) drives hematopoietic stem cell (HSC) differentiation into the myeloid lineage, and enhanced IL-1β signaling plays a key role in hematological malignancies. However, little is known on the role of its endogenous regulatory cytokine, IL-1 receptor antagonist (IL-1rn), on both healthy and malignant hematopoiesis. Here, we show that inflammation through unbalanced IL-1rn is present in the experimental model of acute myeloid leukemia (AML) driven by the NRAS-G12D oncogene.
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| Overall design |
Total RNA was isolated from bone marrow (BM) HSC subsets of Mx1-Cre NRAS-G12D mice (IL-1rn-KO, n=3) and NRAS-G12D control (n=3) mic, 6 weeks after pI:pC injection. HSC were FACS sorted and immunophenotypically defined as follows: lin− c-kit+ Sca-1+ (LSK) CD34- Flt3- long term hematopoietic stem cells (LT-HSC), LSK CD34+ Flt3- short term HSC (ST-HSC) and LSK CD34+ Flt3+ multipotent progenitors (MPP). RNA was amplified and prepared for RNA-Seq using the SMART-Seq v4 Ultra Low Input RNA kit (Clontech).
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| Contributor(s) |
Villatoro AE, Arranz L |
| Citation(s) |
36596811 |
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| Submission date |
Feb 15, 2019 |
| Last update date |
Feb 13, 2023 |
| Contact name |
Lorena Arranz |
| E-mail(s) |
lorena.arranz@medisin.uio.no
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| Phone |
+47 409 95 107
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| Organization name |
University of Oslo
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| Department |
CRESCO - Centre for Embryology and Healthy Development
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| Lab |
Stem Cells, Ageing and Cancer Research Group
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| Street address |
Forskningsveien 1
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| City |
Oslo |
| ZIP/Postal code |
0373 |
| Country |
Norway |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA522750 |
| SRA |
SRP186003 |
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