|
| Status |
Public on Feb 13, 2019 |
| Title |
WRN knockout effects upon gene expression in SW48 and OVK18 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To determine gene expression changes upon CRISPR/Cas9-mediated WRN knockout in SW48 and OVK18. 72 hours after transduction of sgRNA into Cas9 expressing cells, RNA was harvested and submitted for sequencing.
|
| |
|
| Overall design |
Cas9 expressing SW48 and OVK18 cells were transduced with either a negative control sgRNA targeting an intergenic region of chromosome 2 (sgCh2-2), or one of two sgRNAs targeting WRN (sgWRN2, sgWRN3)
|
| |
|
| Contributor(s) |
Chan EM, Shibue T |
| Citation(s) |
30971823 |
| |
| Submission date |
Feb 12, 2019 |
| Last update date |
Apr 15, 2019 |
| Contact name |
Edmond Chan |
| E-mail(s) |
Edmond_Chan@dfci.harvard.edu
|
| Phone |
6262789224
|
| Organization name |
Dana Farber Cancer Institute
|
| Street address |
450 Brookline Ave
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA521984 |
| SRA |
SRP185728 |
Less...
More...