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| Status |
Public on Sep 01, 2019 |
| Title |
Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [mRNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To compare immature and mature platelet transcriptome
Methods: reticulated or immature platelets(RPs) were defined as the platelets with the highest thiazole orange staining intensity (15% highest, RNAhigh) according to previous experiences and mature platelets were defined as the platelet with the 30% lowest thiazole orange staining intensity (RNAlow) and sorted (supplemental Figure S1D).
We detected more than 1700 differentially expressed gene, incluning activation receptors
RPs have a prothrombotic transcriptomic profile
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| Overall design |
Platelets RNA was acquired frome healthy donors. And analyzed using using NextSeq-500
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| Contributor(s) |
Bongiovanni D, Santamaria G |
| Citation(s) |
31473989 |
| |
| Submission date |
Feb 12, 2019 |
| Last update date |
Sep 03, 2019 |
| Contact name |
Gianluca Santamaria |
| E-mail(s) |
santamariagianluca@tum.de
|
| Organization name |
Klinikum rechts der Isar – Technical University Munich
|
| Department |
Department of Medicine I
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| Lab |
Molecular Cardiology
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| Street address |
Ismaninger Strasse 22
|
| City |
Munich |
| State/province |
Germany |
| ZIP/Postal code |
81675 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE126448 |
Transcriptome analysis of reticulated platelets reveals a prothrombotic profile |
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| Relations |
| BioProject |
PRJNA521955 |
| SRA |
SRP185700 |
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