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| Status |
Public on Sep 01, 2019 |
| Title |
Transcriptome analysis of different brain areas in Hcrt gene and Hcrt cell ablated mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Hypocretin (HCRT) and melanin concentrating hormone (MCH) are brain neuropeptides regulating a wide range of functions including sleep and metabolism. Loss of function of HCRT causes the sleep disorder narcolepsy. We found that loss of HCRT neurons in Hcrt-ataxin-3 mice results in a specific 50% decrease in another orexigenic neuropeptide QRFP that might explain the metabolic syndrome in narcolepsy.
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| Overall design |
To identify cell type-specific regulators of HCRT cells, we used two commonly used mouse models of narcolepsy with cataplexy: Hcrt gene knockout (Hcrtko/ko) mice and Hcrt-ataxin-3 mice in which HCRT cells are ablated by the expression of ataxin-3 gene. We hypothesized that comparing the brain transcriptome of these mice and their controls might identify transcriptional changes due both to a lack of Hcrt gene and HCRT neurons altogether (as in narcolepsy).
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| Contributor(s) |
Seifinejad A, Li S, Mikhail C, Vassalli A, Pradervand S, Arribat Y, Modarres HP, Allen B, John RM, Amati F, Tafti M |
| Citation(s) |
31375626 |
| |
| Submission date |
Feb 10, 2019 |
| Last update date |
Sep 18, 2019 |
| Contact name |
Sylvain Pradervand |
| E-mail(s) |
Sylvain.Pradervand@unil.ch
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| Phone |
+41 21 692 39 08
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| Organization name |
UNI Lausanne
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| Department |
CIG
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| Lab |
DNA Array Facility
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| Street address |
Genopode
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (36)
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| Relations |
| BioProject |
PRJNA521660 |
| SRA |
SRP185358 |
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