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| Status |
Public on Mar 04, 2019 |
| Title |
RNAseq analysis of glomerular transcriptome of wild-type vs. Podocyte Cosmc knockout |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cosmc is an obligate chaperone for C1GalT1 T transferase necessary for generating mature mucin-type O-glycans. Animals lacking Cosmc in glomerular podocytes develop proteinuria, glomerulosclerosis and progressive renal failure. This study was designed to examine transcriptome differences in 1 month male animals with and without podocyte Cosmc, a time point when proteinuria is established but glomerular architecture is still relatively intact.
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| Overall design |
Glomeruli were isolated by Dynabead perfusion technique from 1 month old control(n=5) and Podo-Cre; CosmcY/flox(n=4) male animals. RNA was isolation using Rneasy Plus Mini kit (Qiagen). Samples were sent to GeneWiz for polyA-based mRNA enrichment, cDNA synthesis, RNA-seq library preparation and sequencing on an Illumina HiSeq. Paired end 2x150 bp reads were obtained. Bioinformatic analysis was performed through GeneWiz
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| Contributor(s) |
Schlondorff JS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 07, 2019 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Johannes Schlondorff |
| E-mail(s) |
jschlond@bidmc.harvard.edu
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| Phone |
617-667-0508
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| Organization name |
Beth Israel Deaconess Medical Center
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| Department |
Nephrology
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| Street address |
99 Brookline Ave, RN304B
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA521340 |
| SRA |
SRP184497 |
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