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| Status |
Public on Mar 13, 2020 |
| Title |
Bioenergetic control of synaptic plasticity by astrocytes during acute stress |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Aldh1l1CreERT2-RiboTag mice were used to purify and sequence astrocyte actively translated mRNAs from acute stress mouse models (20 minute forced swim) and non stressed controls.
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| Overall design |
Four weeks after tamoxifen injection, somatosensory cortex was homogenized and RNA was purified from (i) cleared lysate as the input and control, and (ii) astrocyte-specific ribosome-associated RNA precipitated via a hemagglutinin (HA) tag. Stress mouse astrocyte transcriptomes were compared to non stressed.
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| Contributor(s) |
Murphy-Royal C, Boyce AK, Diaz-Castro B, Khakh BS, Thompsom RJ, Bains JS, Gordon GR |
| Citation(s) |
32332733 |
| |
| Submission date |
Feb 06, 2019 |
| Last update date |
May 07, 2020 |
| Contact name |
Giovanni Coppola |
| E-mail(s) |
gcoppola@ucla.edu
|
| Phone |
310-794-4172
|
| Organization name |
UCLA
|
| Department |
Psychiatry and Neurology
|
| Lab |
Neurogenetics
|
| Street address |
1524 Gonda, 695 Charles Young Drive South
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA521236 |
| SRA |
SRP184236 |
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