|
| Status |
Public on May 01, 2020 |
| Title |
Distinct fibroblast functional states drive clinical outcomes in ovarian cancer and are regulated by TCF21 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Recent studies indicate that cancer-associated fibroblasts (CAFs) are phenotypically and functionally heterogeneous. However, little is known about CAF subtypes and the roles they play in cancer progression. Here we identify and characterize two CAF subtypes that coexist within high grade serous ovarian cancers: Fibroblast activation protein (FAP)-high (FH) CAFs resemble the classical myofibroblast-type CAF, whereas FAP-low (FL) CAFs possesses a preadipocyte-like molecular signature.
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| |
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| Overall design |
High-grade serous ovarian cancer single cell suspensions of 12 patients were stained for fluorescence activated cell sorting (FACS) CD31-CD45-EpCAM-CD49e+ (CAFs) cells, and CD31-CD45-EpCAM+CD133+ (epithelial) cells were gated for sorting based on FMO controls. Cells were sorted and RNA was extracted immediately using the RNeasy Plus Micro kit (Qiagen).
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| |
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| Contributor(s) |
Ailles LE, Hussain A |
| Citation(s) |
32434219 |
| |
| Submission date |
Feb 05, 2019 |
| Last update date |
Aug 04, 2020 |
| Contact name |
Laurie Ailles |
| E-mail(s) |
lailles@uhnresearch.ca
|
| Phone |
416-581-7868
|
| Organization name |
University Health Network
|
| Department |
Princess Margaret Cancer Centre
|
| Lab |
Ailles Lab
|
| Street address |
101 College St.
|
| City |
Toronto |
| State/province |
ON |
| ZIP/Postal code |
M5G 1L7` |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE126133 |
Distinct fibroblast functional states drive clinical outcomes in ovarian cancer and are regulated by TCF21 |
|
| Relations |
| BioProject |
PRJNA521071 |
| SRA |
SRP183812 |
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