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| Status |
Public on Feb 01, 2019 |
| Title |
Drosophila melanogaster female late third instar larval gonads single-cell RNA-Seq (scRNA-Seq) profiling |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The larval ovary is made up of multiple cell types including germ cells and somatic cells. The diversity of cell types and transcriptional regulation is not fully understood. To get single cell resolution of larval ovary regulation, we generated single-cell RNA expression profiles (scRNA-Seq) from late third instar larval ovaries of a reference Drosophila melanogaster genotype w[1118].
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| Overall design |
We generated scRNA-Seq profiles of late third instar larval ovaries in biological triplicate pools using the 10X Chromium System (10X Genomics, Pleasanton, CA) and sequenced (Read1=26 bp, Read2=98 bp and Readi7=8 bp) on a HiSeq 2500 (Illumina, San Diego, CA).
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| Contributor(s) |
Mahadevaraju S, Fear JM, Oliver B |
| Citation missing |
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| Submission date |
Jan 31, 2019 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Justin Matthew Fear |
| E-mail(s) |
justin.fear@nih.gov
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| Phone |
301-435-8405
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| Organization name |
NIDDK, NIH
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| Department |
LCDB
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| Lab |
Developmental Genomics
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| Street address |
50 South Drive
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (3) |
| GSM3585578 |
Third instar larval ovary dissociated with Trypsin and Collagenase rep 1 |
| GSM3585579 |
Third instar larval ovary dissociated with Trypsin and Collagenase rep 2 |
| GSM3585580 |
Third instar larval ovary dissociated with Trypsin and Collagenase rep 3 |
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| This SubSeries is part of SuperSeries: |
| GSE125949 |
Single-cell RNA-seq of Drosophila melanogaster male and female late third instar larval gonads |
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| Relations |
| BioProject |
PRJNA518742 |
| SRA |
SRP183201 |
