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Series GSE125914 Query DataSets for GSE125914
Status Public on Aug 06, 2019
Title Calcineurin promotes myogenic gene expression
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We identified four major temporal patterns of Calcineurin (Cn)-dependent gene expression in differentiating myoblasts and determined that Cn is broadly required for the activation of the myogenic gene expression program. Cn promotes gene expression through direct binding to myogenic promoter sequences and facilitating the binding of BRG1 and other SWI/SNF subunit proteins as well as the binding of MyoD, a critical lineage determinant for skeletal muscle differentiation. We conclude that the Cn phosphatase directly impacts the expression of myogenic genes by promoting ATP-dependent chromatin remodeling and formation of transcription-competent promoters.
Overall design Gene expression profiles of C2C12 cells treated with DMSO or FK506 at four time points growth, 0h, 24h, 72h in differentiation.
Contributor(s) Nogami J, Maehara K, Ohkawa Y, Imbalzano AN
Citation(s) 31308130
Submission date Jan 30, 2019
Last update date Oct 01, 2019
Contact name Yasuyuki Ohkawa
Organization name Medical Institute of Bioregulation
Lab Division of Transcriptomics
Street address 3-1-1 Maidashi
City Fukuoka
ZIP/Postal code 8128582
Country Japan
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (39)
GSM3584879 GM-DMSO rep1
GSM3584880 GM-DMSO rep2
GSM3584881 GM-DMSO rep3
BioProject PRJNA517941
SRA SRP182961

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Supplementary file Size Download File type/resource
GSE125914_umi_counts.txt.gz 790.4 Kb (ftp)(http) TXT
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