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Series GSE125865 Query DataSets for GSE125865
Status Public on Jan 31, 2020
Title mRNA destabilization by BTG1 and BTG2 maintains T cell quiescence [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Quiescence is a cellular state in which cells undergo reversible cell cycle arrest in response to environmental challenges or lack of stimuli. When favorable conditions are engaged, the cells exit quiescence and start to proliferate. For example, quiescent stem cells and oocytes are activated by differentiation signals and fertilization, respectively. The regulation of quiescent state is also crucial for lymphocytes, such as T cells. Inappropriate activation of T cells often leads to autoimmune diseases and thus, the balance between quiescent and activated T cells must be preserved. Despite its importance, however, how T cells maintain the ‘quiescent state’ and exit from it remain largely unknown. Here, we identify BTG1 and BTG2 (BTG1/2) as factors responsible for T cell quiescence. Knockout of Btg1/2 in T cells resulted in a reduced threshold to T cell activation, increased proliferation and a concomitant reduction of the frequency of naive T cells. Loss of BTG1/2 rendered naive T cells to become spontaneously activated even without TCR stimuli. Interestingly, we found a global increase in mRNA abundance in naive T cells that lack BTG1/2. In addition, depletion of BTG1/2 led to an increase in mRNA half-life and poly(A) tail length. Thus, BTG1/2 promotes deadenylation and degradation of mRNA in naive T cells. Our study demonstrates a key mechanism underlying T cell quiescence, and suggests low mRNA abundance as a key feature for maintaining quiescent T cells.
Overall design Two replicates of naive CD4 T cells (TCRb+CD4+CD25-CD62LhiCD44low) from WT and DKO mice, respectively.
Contributor(s) Lim J, Hwang S, Flavell RA
Citation(s) 32165587
Submission date Jan 29, 2019
Last update date Mar 15, 2020
Contact name Jaechul Lim
Organization name Seoul National University
Street address 1 Gwanak-ro, 1 Gwanak-gu
City Seoul
State/province CT
ZIP/Postal code 08826
Country South Korea
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (4)
GSM3583641 RNA-seq WT1
GSM3583642 RNA-seq WT2
GSM3583643 RNA-seq DKO1
This SubSeries is part of SuperSeries:
GSE125890 mRNA destabilization by BTG1 and BTG2 maintains T cell quiescence
BioProject PRJNA517726
SRA SRP182851

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Supplementary file Size Download File type/resource
GSE125865_RAW.tar 6.3 Mb (http)(custom) TAR (of TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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