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| Status |
Public on Aug 20, 2019 |
| Title |
Niche-specific factors dynamically regulate sebaceous gland stem cells during skin homeostasis and aging |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We deleted different Notch pathway components in different sub-compartments of mouse skin, and performed RNA-seq to assess differentially regulated genes, 10 weeks after deletion.
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| Overall design |
Mice were injected with tamoxifen at 8 weeks of age to delete either Rbpj (R) or Notch1 (N) in the skin. Cre drivers were K14-CreERT (K) and Lrig1-CreERT2 (L). Dorsal skin in telogen phase was harvested, isolated away from dermis, and extracted for total RNA by Qiagen RNeasy kit, 10 weeks post-tamoxifen. LR = Lrig1-CreERT2+Rbpj-deletion; KR = K14-CreERT+Rbpj-deletion; N = K14-CreERT1+Notch1-deletion. All 3 mutant strains had matched controls (Cont) from the same batch/litter. See Veniaminova, Wong et al., 2019
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| Contributor(s) |
Sunny W, Lam T |
| Citation(s) |
31564613 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AR065409 |
The Hair Follicle Infundibulum: A Potential Mediator of Skin Barrier Function |
REGENTS OF THE UNIVERSITY OF MICHIGAN - ANN ARBOR |
Sunny Wong |
| K01 AR072129 |
Integrative Biology Approach to Identify and Characterize Roles of lncRNAs Associated with Psoriasis Pathology |
REGENTS OF THE UNIVERSITY OF MICHIGAN - ANN ARBOR |
Lam Cheung Tsoi |
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| Submission date |
Jan 28, 2019 |
| Last update date |
Nov 19, 2019 |
| Contact name |
Lam Tsoi |
| E-mail(s) |
alextsoi@umich.edu
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| Organization name |
University of Michigan
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| Street address |
Medical Sciences 1 Bldg. 1301 E. Catherine
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| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (18)
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| Relations |
| BioProject |
PRJNA517423 |
| SRA |
SRP182655 |
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