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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 26, 2019 |
| Title |
Two distinct interstitial macrophage populations coexist across tissues in unique subtissular niches [FACs sorted] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Macrophages are a heterogeneous cell population involved in tissue homeostasis, inflammation and in multiple pathologies. Although the major tissue-resident macrophage populations have been extensively studied, interstitial macrophages (IMs) residing within tissue parenchyma remain poorly defined. Here, we studied IMs from murine lung, fat, heart and dermis. We identified two independent IM subpopulations that are conserved across tissues: Lyve1loMHCIIhiCX3CR1hi (Lyve1loMHCIIhi) and Lyve1hiMHCIIloCX3CR1lo (Lyve1hiMHCIIlo) monocyte-derived IMs, with distinct gene expression profiles, phenotypes, functions, and localisation. Using a mouse model of inducible macrophage depletion (SLCO2B1-DTR), we found that the absence of Lyve1hiMHCIIlo IMs exacerbated experimental lung fibrosis. Thus, we demonstrate that two independent populations of IMs exist across tissues and exhibit conserved niche-dependent functional programming.
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| Overall design |
FACS sorted cells from several animals
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| Contributor(s) |
Chakarov S, Lim HY, Tan L, Lim SY, See P, Lum J, Zhang X, Foo S, Nakamizo S, Kaibo D, Kong WT, Gentek R, Balachander A, Carbajo D, Bleriot C, Malleret B, Tam JK, Baig S, Shabeer M, Toh SE, Schlitzer A, Larbi A, Marichal T, Malissen B, Chen J, Poidinger M, Kabashima K, Bajenoff M, Ng LG, Angeli V, Ginhoux F |
| Citation(s) |
30872492 |
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| Submission date |
Jan 25, 2019 |
| Last update date |
Apr 27, 2019 |
| Contact name |
Florent Ginhoux |
| E-mail(s) |
Florent_Ginhoux@immunol.a-star.edu.sg
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| Organization name |
Singapore Immunology Network (SIgN), A*STAR
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| Street address |
8A Biomedical Grove, Immunos Building, Level 3
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| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (31)
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| This SubSeries is part of SuperSeries: |
| GSE125691 |
Two distinct interstitial macrophage populations coexist across tissues in unique subtissular niches |
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| Relations |
| BioProject |
PRJNA517143 |
| SRA |
SRP182065 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE125667_RAW.tar |
9.1 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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