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| Status |
Public on Mar 07, 2019 |
| Title |
Transcriptome-wide profiling of thyroid hormone-dependent gene expression in the mouse heart |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
During the first weeks after birth, cardiomyocytes within the mouse heart progressively exit the cell cycle, binucleate, and lose regenerative capacity. We have determined that postnatal day 14 (P14) mouse hearts incapable of responding to thryoid hormone signaling have enhanced proliferation, retain greater populations of mononucleated cardiomyocytes, and show increased regenerative ability. In this study, we perform transcriptome-wide analysis to identify gene networks regulating thryoid hormone-dependent exit of cardiomyocyte cell-cycle and loss of regenerative potential.
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| Overall design |
Transgenic mice possessing cardiomyocyte-specific expression of Cre recombinase (Myh6-Cre) were bred with knock-in mice harboring an allele with Cre-dependent expression of a dominant negative mutant form of thryoid hormone receptor alpha (TRalphaAMI). Three Myh6-Cre; TRalphaAMI pups and three TRalphaAMI littermate control pups were raised to postnatal day 14 (P14), total RNA was extracted from their hearts, and RNA-seq was performed to identify differentially expressed genes.
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| Contributor(s) |
Huang GN |
| Citation(s) |
30846611 |
| |
| Submission date |
Jan 24, 2019 |
| Last update date |
Mar 27, 2019 |
| Contact name |
Guo N Huang |
| E-mail(s) |
Guo.Huang@ucsf.edu
|
| Phone |
415-502-2873
|
| Organization name |
University of California, San Francisco
|
| Department |
Cardiovascular Research Institute
|
| Lab |
Huang
|
| Street address |
555 Mission Bay Blvd S, CVRB 352V
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA516894 |
| SRA |
SRP181889 |
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