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| Status |
Public on Dec 31, 2008 |
| Title |
Transcript Analysis of E. coli ATCC 35218 suggest a Role of cranberry Juice in Inhibiting the growth of E. coli |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
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| Summary |
Several different mechanisms have been proposed to explain the possible role of cranberries, cranberry juice, and cranberry extracts in inhibiting bacterial growth. In this report, we showed that Escherichia coli showed slower growth rate in response to the presence of cranberry juice in the growth media. By compareing the global transcript profiles, significant modulation of several genes of E. coli grown in LB broth with 10% cranberry juice were identified and provided identification of the potential mechanisms involved in the inhibitory effects of cranberry juice. The results presented clearly demonstrate that the inhibitory effect on bacterial growth observed in the presence of cranberry juice/extracts is primarily a result of the iron chelation capacity of PACs and direct disruption of metabolic enzymes. The results are discussed with a focus on the genes associated with iron chelation capability.
Keywords: growth inhibition of cranberry juice
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| Overall design |
For transcriptome profiling, there were 15 Affymetrix GeneChip® E. coli genome 2.0 arrays total. There were five conditions: E. coli grown in LB broth, E. coli grown in LB broth with 10% cranberry juice to generation 50, 160, 210, and 230. Each condition was done in triplicate. Five conditions done in triplicates resulted in 15 samples that went onto 15 microarrays.
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| Contributor(s) |
Lin B, Johnson BJ, Rubin RA, Malanoski AP, Ligler FS |
| Citation(s) |
18957286 |
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| Submission date |
Aug 26, 2008 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Baochuan Lin |
| E-mail(s) |
baochuan.lin@nrl.navy.mil
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| Phone |
202-767-0289
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| Organization name |
US Naval Research Laboratory
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| Department |
Center for Bio/Molecular Science & Engineering
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| Street address |
4555 Overlook Avenue, S. W.
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| City |
Washington |
| State/province |
DC |
| ZIP/Postal code |
20375 |
| Country |
USA |
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| Platforms (1) |
| GPL3154 |
[E_coli_2] Affymetrix E. coli Genome 2.0 Array |
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| Samples (15)
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| GSM315527 |
Generation 50 E. coli grown in LB broth + 10% cranberry juice, rep 1 |
| GSM315528 |
Generation 50 E. coli grown in LB broth + 10% cranberry juice, rep 2 |
| GSM315529 |
Generation 50 E. coli grown in LB broth + 10% cranberry juice, rep 3 |
| GSM315530 |
Generation 160 E. coli grown in LB broth + 10% cranberry juice, rep 1 |
| GSM315531 |
Generation 160 E. coli grown in LB broth + 10% cranberry juice, rep 2 |
| GSM315532 |
Generation 160 E. coli grown in LB broth + 10% cranberry juice, rep 3 |
| GSM315533 |
Generation 210 E. coli grown in LB broth + 10% cranberry juice, rep 1 |
| GSM315534 |
Generation 210 E. coli grown in LB broth + 10% cranberry juice, rep 2 |
| GSM315535 |
Generation 210 E. coli grown in LB broth + 10% cranberry juice, rep 3 |
| GSM315536 |
Generation 230 E. coli grown in LB broth + 10% cranberry juice, rep 1 |
| GSM315537 |
Generation 230 E. coli grown in LB broth + 10% cranberry juice, rep 2 |
| GSM315538 |
Generation 230 E. coli grown in LB broth + 10% cranberry juice, rep 3 |
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| Relations |
| BioProject |
PRJNA112623 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12554_RAW.tar |
11.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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