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Series GSE125320 Query DataSets for GSE125320
Status Public on Jan 19, 2019
Title Maintenance of nucleolar homeostasis by CBX4 alleviates senescence and osteoarthritis
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary CBX4, a component of polycomb repressive complex 1 (PRC1), plays important roles in the maintenance of cell identity and organ development through epigenetic silencing. However, whether CBX4 regulates the homeostasis of human stem cells remains unclear. Here, we demonstrate that CBX4 counteracts human mesenchymal stem cell (hMSC) aging via the maintenance of nucleolar homeostasis. CBX4 protein is decreased in aged hMSCs, and targeted CBX4 knockout in young hMSCs results in destabilized nucleolar heterochromatin, increased ribosome biogenesis and protein translation, and accelerated cellular senescence. CBX4 maintains nucleolar homeostasis by recruiting nucleolar protein fibrillarin and heterochromatin organization associated protein KAP1 at nucleolar rDNA, limiting the excessive expression of rRNAs. Importantly, overexpression of CBX4 alleviates physiological hMSC aging and attenuates the development of posttraumatic osteoarthritis in mice. Taken together, our findings reveal a novel role of CBX4 in counteracting senescence by maintaining nucleolar homeostasis, providing a potential therapeutic target for aging-associated disorders.
Overall design Transcriptome analysis of knee joints tissues upon the treatment of lentiviruses expressing Luc, CBX4 in anterior cruciate ligament transection (ACLT) induced osteoarthritis (OA) mouse model.
Contributor(s) Liu G
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Submission date Jan 18, 2019
Last update date Mar 01, 2019
Contact name Zunpeng Liu
Phone +86 15510715055
Organization name State Key Laboratory of Stem Cell and Reproductive Biology
Department Institute of Zoology, Chinese Academy of Sciences
Lab Jing Qu
Street address Beichen West Road
City Beijing
ZIP/Postal code 100101
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (9)
GSM3568915 OA model_Non surgery rep1
GSM3568916 OA model_Non surgery rep2
GSM3568917 OA model_Non surgery rep3
BioProject PRJNA515848
SRA SRP180201

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Supplementary file Size Download File type/resource
GSE125320_RAW.tar 1.7 Mb (http)(custom) TAR (of TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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