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| Status |
Public on Nov 19, 2019 |
| Title |
The Nuclear Matrix Protein SAFB Cooperates with Major Satellite RNAs to Stabilize Heterochromatin Architecture Partially through Phase Separation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Interphase chromatin is hierarchically organized into higher-order architectures that are essential for gene functions, yet the biomolecules that regulate these 3D architectures remain poorly understood. Here, we show that scaffold attachment factor B (SAFB), a nuclear matrix (NM)-associated protein with RNA-binding functions, modulates chromatin condensation and stabilizes heterochromatin foci in mouse cells. SAFB interacts via its R/G-rich region with heterochromatin-associated repeat transcripts such as major satellite RNAs, which promote the phase sep- aration driven by SAFB. Depletion of SAFB leads to changes in 3D genome organization, including an increase in interchromosomal interactions adjacent to pericentromeric heterochromatin and a decrease in genomic compartmentalization, which could result from the decondensation of pericentromeric heterochromatin. Collectively, we reveal the integrated roles of NM-associated proteins and repeat RNAs in the 3D organization of heterochromatin, which may shed light on the molecular mechanisms of nuclear architecture organization.
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| Overall design |
Refer to individual Series
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| |
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| Citation(s) |
31677973 |
| |
| Submission date |
Jan 14, 2019 |
| Last update date |
Jan 30, 2020 |
| Contact name |
Bo Wen |
| E-mail(s) |
bowen75@fudan.edu.cn
|
| Organization name |
Fudan University
|
| Street address |
130 Dongan Rd.
|
| City |
ShangHai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platforms (2) |
| GPL10448 |
Agilent-027414 SurePrint G3 Mouse CGH Microarray 1x1M |
| GPL21273 |
HiSeq X Ten (Mus musculus) |
|
| Samples (28)
|
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| This SuperSeries is composed of the following SubSeries:
|
| GSE141080 |
The nuclear matrix protein SAFB maintains heterochromatin architecture through RNA-dependent phase separation [Hi-C] |
| GSE141084 |
The nuclear matrix protein SAFB maintains heterochromatin architecture through RNA-dependent phase separation [RIP-seq] |
| GSE141104 |
The nuclear matrix protein SAFB maintains heterochromatin architecture through RNA-dependent phase separation [H3K9me3 ChIP-seq] |
| GSE141109 |
Genome-wide maps of nuclear lamina interactions in AML12 cells upon Safb KD [DamID] |
| GSE141113 |
The nuclear matrix protein SAFB maintains heterochromatin architecture through RNA-dependent phase separation [HiChIP] |
| GSE141294 |
The Nuclear Matrix Protein SAFB Cooperates with Major Satellite RNAs to Stabilize Heterochromatin Architecture Partially through Phase Separation [RNA-seq] |
|
| Relations |
| BioProject |
PRJNA515050 |
| SRA |
SRP180874 |
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