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Series GSE124995 Query DataSets for GSE124995
Status Public on Jan 12, 2019
Title Next-generation sequencing of murine trophoblast-derived and pregnancy-associated exosome-enriched extracellular vesicle microRNAs
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary The role of extracellular vesicles (EVs), specifically exosomes, in intercellular communication likely plays a key role in placental orchestration of pregnancy and maternal immune sensing of the fetus. While murine models are powerful tools to study pregnancy and maternal-fetal immune interactions, in contrast to human placental exosomes, the content of murine placental and pregnancy exosomes remains largely understudied. Using a recently developed in vitro culture technique, murine trophoblast stem cells derived from B6 mice were differentiated into syncytial-like cells. EVs from the conditioned media, as well as from pregnant and non-pregnant sera, were enriched for exosomes. The RNA composition of these murine trophoblast-derived and pregnancy-associated exosome-enriched-EVs (ExoE-EVs) was determined using RNA-sequencing analysis and expression levels confirmed by qRT-PCR. Differentially abundant miRNAs were detected in syncytial differentiated ExoE-EVs, particularly from the X chromosome cluster (mmu-miR-322-3p, mmu-miR-322-5p, mmu-miR-503-5p, mmu-miR-542-3p, and mmu-miR-450a-5p). These were confirmed to be increased in pregnant mouse sera ExoE-EVs by qRT-PCR analysis. Interestingly, fifteen miRNAs were only present within the pregnancy-derived ExoE-EVs compared to non-pregnant controls. Mmu-miR-292-3p and mmu-miR-183-5p were noted to be some of the most abundant miRNAs in syncytial ExoE-EVs and were also present at higher levels in pregnant versus non-pregnant sera ExoE-EVs. The bioinformatics tool, MultiMir, was employed to query publicly available databases of predicted miRNA-target interactions. This analysis reveals that the X-chromosome miRNAs are predicted to target ubiquitin-mediated proteolysis and intracellular signaling pathways. Knowing the cargo of placental and pregnancy-specific ExoE-EVs as well as the predicted biological targets informs studies using murine models to examine not only maternal-fetal immune interactions but also the physiologic consequences of placental-maternal communication.
Overall design Examination of EVs isolated from 1) conditioned media from in vitro mouse trophoblast cells cultures maintained as "STEM" using Cell Start and FGF4 (n=3), 2) conditioned media from in vitro mouse trophoblast cultures that have undergone syncytial-like differentiation (n=3), 3) non-pregnant B6 mouse sera (n=3); 4) pregnant B6 mouse sera (gestational day 14.5, n=3). EVs were isolated using ExoQuick-TC (1-2) or ExoQuick (3-4).
Contributor(s) Stefanski AL, Martinez N, Peterson LK, Callahan TJ, Treacy E, Luck M, Friend SF, Hermesch A, Maltepe E, Phang T, Dragone LL, Winn VD
Citation(s) 30730971
Submission date Jan 11, 2019
Last update date Mar 06, 2019
Contact name Virginia D. Winn
Organization name Stanford University
Department OB-GYN/Reproductive, Perinatal and Stem Cell Biology Research
Street address 300 Pasteur Drive, Boswell A364
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
Platforms (1)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (12)
GSM3560727 Stem-A
GSM3560728 Stem-B
GSM3560729 Stem-C
BioProject PRJNA514778
SRA SRP178560

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Supplementary file Size Download File type/resource
GSE124995_Abundances_Day14.5.txt.gz 9.3 Mb (ftp)(http) TXT
GSE124995_Abundances_Diff.txt.gz 10.1 Mb (ftp)(http) TXT
GSE124995_Abundances_NP.txt.gz 9.1 Mb (ftp)(http) TXT
GSE124995_Abundances_Stem.txt.gz 10.3 Mb (ftp)(http) TXT
GSE124995_NPvsDay14.5_all_with_type.1384366252915.tsv.gz 9.9 Mb (ftp)(http) TSV
GSE124995_StemvsDiff_all_with_type.1384366250358.tsv.gz 13.2 Mb (ftp)(http) TSV
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Raw data are available in SRA
Processed data are available on Series record

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