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| Status |
Public on Jan 08, 2019 |
| Title |
Gene expression in melanoma cells following treatment with IFN-beta |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To further determine the candidate genes which expressed in four melanoma cells and melanocyte after treatment with IFN-β, total RNA was extracted from melanoma cells, which have different sensitivities to IFN-β, and melanocyte after treatment with or without IFN-β at a concentration of 1,000 IU/ml for 48 h. For expression profiling, oligonucleotide microarray analysis was performed at Hokkaido System Science (Sapporo, Japan) using an Agilent Human Genomic microarray 8 × 60 K version 2.0 (Agilent Technologies, Santa Clara, CA). Then, we focused on two candidate genes, CXCL-10 and IL-24, which are associated with the sensistibity of melanoma cells to IFN-β. We examined whether CXCL10 and IL-24 directly or indirectly cause sensitivitiy of melanoma to IFN-β.
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| Overall design |
total RNA was extracted from melanoma cells (A375, RPMI-7951, SK-MEL-5, and SK-MEL-1) and primary melanocyte after treatment with or without IFN-β at a concentration of 1,000 IU/ml for 48 h.
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| Contributor(s) |
Nobeyama Y, Watanabe Y |
| Citation(s) |
31070806 |
| Submission date |
Jan 07, 2019 |
| Last update date |
May 21, 2019 |
| Contact name |
Yoshinori Watanabe |
| E-mail(s) |
watanabeyoshinori0528@gmail.com
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| Phone |
+81334331111
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| Organization name |
Jikei Hospital
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| Street address |
3-19-18, Nishi-shimbashi
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| City |
Minato-ku |
| State/province |
Tokyo |
| ZIP/Postal code |
1050003 |
| Country |
Japan |
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| Platforms (1) |
| GPL16699 |
Agilent-039494 SurePrint G3 Human GE v2 8x60K Microarray 039381 (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA513347 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE124759_RAW.tar |
84.3 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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