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| Status |
Public on Oct 20, 2020 |
| Title |
Blood RNA signature predicts recent tuberculosis exposure in mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Knowing when a person was infected with Mycobacterium tuberculosis (M.tb) is critical as recent infection is the strongest clinical risk factor for progression to TB disease in immunocompetent individuals. However, time since M.tb infection is very difficult to determine in routine clinical practice. We determined whether gene expression patterns in blood RNA correlate with time since M.tb infection or exposure to develop a biomarker for recent exposure. As proof of concept, we found an RNA signature that accurately discriminated early and late time periods after experimental infection in mice.
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| Overall design |
Blood was collected from 20 C57BL/6 mice infected with a low dose of M.tb (Erdman), as well as 6 uninfected age-matched control mice. Blood was collected at days 30, 60, 90, 120 and 150 post-infection. Whole blood was subjected to microarray analysis. We identify a gene expression signature that predicts infection time point.
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| Contributor(s) |
Ault RC, Headley CA, Hare AE, Carruthers BJ, Mejias A, Turner J |
| Citation(s) |
33037303 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 AI064522 |
Immune correlates of reactivation tuberculosis |
OHIO STATE UNIVERSITY |
JOANNE TURNER |
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| |
| Submission date |
Jan 04, 2019 |
| Last update date |
Oct 20, 2020 |
| Contact name |
Joanne Turner |
| E-mail(s) |
joanneturner@txbiomed.org
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| Organization name |
Texas Biomedical Research Institute
|
| Street address |
8715 W Military Dr
|
| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78227 |
| Country |
USA |
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| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA513976 |
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