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| Status |
Public on Nov 19, 2019 |
| Title |
Transcriptomic responses in mouse blood during the first week after in vivo gamma irradiation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Due to limitations of available human models for development of gene expression based radiation biodosimetry, many such studies have made use of mouse models. To provide a broad view of the gene expression response to irradiation in the mouse, we have exposed male C57BL/6 mice to 0, 1.5, 3, 6 or 10 Gy of gamma rays, sacrificing groups of the mice at 1, 2, 3, 5, or 7 days after exposure. Global gene expression was then profiled in blood from individual mice using Agilent microarrays. In general, we found increasing numbers of genes differentially expressed with increasing dose, with more prolonged responses after the higher doses.
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| Overall design |
Male C57BL/6 mice were either sham irradiated as controls or exposed to 1.5, 3, 6 or 10 Gy of gamma-rays. Mice from each dose group (including controls) were sacrificed at either 1, 2, 3, 5 or 7 days after exposure, and blood collected for microarray analysis. Ten biological replicates, representing independent mice, were done for each dose and time point, with the exception of the 10Gy dose on day 7, for which only 8 replicates were obtained.
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| Contributor(s) |
Amundson SA, Paul S |
| Citation(s) |
31797975 |
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| Submission date |
Jan 03, 2019 |
| Last update date |
Dec 17, 2019 |
| Contact name |
Sally Amundson |
| E-mail(s) |
saa2108@cumc.columbia.edu
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| Organization name |
Columbia University
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| Department |
Center for Radiological Research
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| Street address |
630 W. 168th St
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (248)
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| Relations |
| BioProject |
PRJNA512841 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE124612_RAW.tar |
2.2 Gb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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