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Series GSE124540 Query DataSets for GSE124540
Status Public on Jun 22, 2021
Title ChIP-seq and RNA-seq analysis of histone Kcr in the embryonic forebrain and neural stem/progenitor cells.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary To gain a deeper insight into how histone lysine crotonylation regulates embryonic neural stem/progenitor cells (NSPCs) proliferation and differentiation, ChIP-seq and RNA-seq were performed to analyze the genome-wide changes of histone Kcr and transcriptiome changes stimulated with crotonate and MS-275 in E13.5 NSPCs.
Overall design Total RNA was extracted from E13.5 NSPCs before and after crotonate and MS-275 stimulation and generated by deep sequencing, in four biological replicates, using Illumina novaseq 6000. ChIP was excuted using pan-Kcr and Pan-Kac antibody with E13.5 NSPCs before and after crotonate and MS-275 stimulation.
Contributor(s) Dai S, Liu C
Citation(s) 34369651
Submission date Jan 02, 2019
Last update date Aug 10, 2021
Contact name Dai Shang Kun
Organization name Shandong University of Technology
Department School of Life Sciences and Medicine
Lab Laboratory of Developmental and Evolutionary Biology
Street address 266 Xincun West Road
City Zibo
State/province Shandong
ZIP/Postal code 255000
Country China
Platforms (2)
GPL23479 BGISEQ-500 (Mus musculus)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (30)
GSM3536156 Transcriptome_E13.5_NSPCs_Control-rep1_RNA-seq
GSM3536157 Transcriptome_E13.5_NSPCs_Control-rep2_RNA-seq
GSM3536158 Transcriptome_E13.5_NSPCs_Control-rep3_RNA-seq
BioProject PRJNA512461
SRA SRP174993

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Supplementary file Size Download File type/resource
GSE124540_E13.5_Brain_Explant_RNA-seq_Crotonate-Control_DEG.txt.gz 1.9 Mb (ftp)(http) TXT
GSE124540_H3K9ac_E13.5_Forebrain_Peaks.bed.gz 417.3 Kb (ftp)(http) BED
GSE124540_H3K9cr_E13.5_Forebrain_Peaks.bed.gz 282.4 Kb (ftp)(http) BED
GSE124540_Histone-Kac_Control_E13.5_NSPCs_Peaks.bed.gz 184.4 Kb (ftp)(http) BED
GSE124540_Histone-Kac_Crotonate_E13.5_NSPCs_Peaks.bed.gz 172.6 Kb (ftp)(http) BED
GSE124540_Histone-Kac_MS-275_E13.5_NSPCs_Peaks.bed.gz 62.1 Kb (ftp)(http) BED
GSE124540_Histone-Kcr_Control_E13.5_NSPCs_Peaks.bed.gz 140.8 Kb (ftp)(http) BED
GSE124540_Histone-Kcr_Crotonate_E13.5_NSPCs_Peaks.bed.gz 184.5 Kb (ftp)(http) BED
GSE124540_Histone-Kcr_MS-275_E13.5_NSPCs_Peaks.bed.gz 48.9 Kb (ftp)(http) BED
GSE124540_RAW.tar 197.8 Mb (http)(custom) TAR (of BW)
GSE124540_RNA-seq_TPM.txt.gz 1.4 Mb (ftp)(http) TXT
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