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Series GSE124532 Query DataSets for GSE124532
Status Public on Nov 12, 2019
Title Transcriptome and DNA methylome of mouse Müller glia
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Müller cells are the primary glia of the neural retina and play crucial roles in maintaining the structural and functional homeostasis of the retina. Müller cells also possess certain levels of retinal regeneration capacity, especially in lower vertebrates. In this study, we deep sequenced the transcriptomes and methylomes of mouse Müller cells and early retinal progenitor cells (RPCs), as well as Müller cells from injured retinas and aged retinas, which will help to reveal molecular mechanisms governing Müller cells development, physiological functions and regeneration activities.
Overall design Total RNAs were extracted and deep sequenced (2 biological replicates each for young Müller cells and aged Müller cells, 3 replicates each for NMDA-treated Müller cells and RPCs). Methylomes of genomic DNAs were deep sequenced using RRBS method (3 biological replicates each for young Müller cells, NMDA-treated Müller cells, aged Müller cells, and RPCs).
Contributor(s) Chen S
Citation(s) 31652328
Submission date Jan 01, 2019
Last update date Nov 14, 2019
Contact name Shuyi Chen
Organization name Zhongshan Ophthlamic Center
Street address #7 Jinsui Rd, Zhujiang Newtown
City Guangzhou
ZIP/Postal code 510623
Country China
Platforms (1)
GPL21273 HiSeq X Ten (Mus musculus)
Samples (22)
GSM3535946 RNA-Mü-1
GSM3535947 RNA-Mü-2
GSM3535948 RNA-Mü-NMDA-1
BioProject PRJNA512399
SRA SRP174977

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE124532_RAW.tar 207.5 Mb (http)(custom) TAR (of TXT)
GSE124532_all.counts.txt.gz 523.2 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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