 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 25, 2019 |
| Title |
Transcriptome of human coronory endothelial cells in response to nitro-conjugated linoleic acid |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In the present study, a distinct transcriptome regulated by NO2-CLA was revealed in primary human coronary artery endothelial cells (HCAECs) through RNA sequencing. Differential gene expression and pathway enrichment analysis identified numerous regulatory networks of cell cycle regulation, inflammation and redox responses, nitric oxide biosynthesis, hypoxia and chaperones in response to NO2-CLA, suggesting potent and diverse effects of NO2-CLA on cellular processes.
|
| |
|
| Overall design |
For RNA-seq experiment, HCAECs at 90% confluence were treated with DMSO, CLA (10µM) or NO2-CLA (10µM) for 1 hour, followed by adding with either ethanol (Control) or PA (200 µM) (Cayman Chemical) for the subsequent 6 hours. There are 6 groups for RNA seq: DMSO + control (ethanol), CLA (10µM) + control, NO2-CLA (10µM) + control, DMSO + PA, CLA (10µM) + PA, NO2-CLA (10µM) + PA. Each group has 4 biological replicates.
|
| |
|
| Contributor(s) |
Lu H, Sun J, Fan Y |
| Citation(s) |
31074702 |
| |
| Submission date |
Dec 31, 2018 |
| Last update date |
Jul 25, 2019 |
| Contact name |
Haocheng Lu |
| E-mail(s) |
lhaochen@umich.edu
|
| Phone |
7347303682
|
| Organization name |
University of Michigan
|
| Department |
Internal Medicine
|
| Lab |
Y. Eugene Chen
|
| Street address |
2800 plymouth road
|
| City |
Ann Arbor |
| State/province |
Michigan |
| ZIP/Postal code |
48105 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA512287 |
| SRA |
SRP174940 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE124522_RAW.tar |
59.2 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
|
|
|
|
 |
Less...
More...