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| Status |
Public on Feb 28, 2019 |
| Title |
B lymphocyte alterations accompany abatacept resistance in new-onset type 1 diabetes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Peripheral blood samples were collected from subjects enrolled in the TrialNet study TN-09. This was a phase II study of the effects of the T cell costimulation inhibitor CTLA4-Ig (abatacept) in new-onset T1D. Total RNA was isolated from whole blood samples and then globin-reduced. RNAseq libraries were prepared from the globin-reduced RNA.
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| Overall design |
The abatacept trial was a randomized, double-blind study of patients (N = 112) with newly diagnosed T1D assigned to receive infusions of CTLA4-Ig (abatacept) or placebo on on days 1, 14, and 28, and then every 28 days with the last dose on day 700. The primary outcome was the baseline-adjusted geometric mean of the 2-h area-under-the-curve (AUC) serum C-peptide concentration after a mixed-meal tolerance test at 2 years’ follow-up. We obtained RNA-seq data from a subset of subjects in the original trial (N = 105 subjects originally), sampled at different visits (0, 84, and 728 days) for a total of 286 samples (mean 2.7 samples per patient). Of 286 initial samples, 272 yielded high-quality RNA-seq data consistent with subject annotation, and were used in downstream analyses.
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| Web link |
https://doi.org/10.1172/jci.insight.126136
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| Contributor(s) |
Linsley PS, Greenbaum CJ, Speake C, Long SA, Dufort MJ |
| Citation(s) |
30830871 |
| |
| Submission date |
Dec 21, 2018 |
| Last update date |
Mar 06, 2024 |
| Contact name |
Stephanie Osmond |
| E-mail(s) |
sosmond@benaroyaresearch.org
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| Organization name |
Benaroya Research Institute
|
| Street address |
1201 9th Ave
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| City |
Seattle, |
| State/province |
WA |
| ZIP/Postal code |
98101 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (286)
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| Relations |
| BioProject |
PRJNA511467 |
| SRA |
SRP174223 |
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